LOCUS       sequence-126401-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY010 (pcDNA3.1-hAsCpf1)
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..115)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(122..169)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccgccactg tgctggatat ctgcagaatt cttaggcata gtcggggaca tcatatgggt
       61 atgcataatc aggcacgtcg tagggataag cgtaatctgg aacatcgtat gggtaggatc
      121 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg ttgcgcagct
      181 cctggatgta ggccagccag tcctgattgg agatgccgtt ctgcagcttc agatccttgc
      241 tctccttcag gtgattcagc agcagctggc ccttcagggc gatgtggtag gcgccattgg
      301 catcggcgtc catgggccac tctgggttct gaaaccggga gtcgaagcac acgccattca
      361 gatcgcgcac ggggctgttg atatagtcct cgcctgtggc ggcattggag ttccgcatct
      421 gcagcacgct gcggatcagg gccaccatgg tgtcgatggc gtgagaatcg tcattctcca
      481 gcagctttgg caggatgttg gagccatccc tgaacacgat gcccttctcc tccagcaggg
      541 cgatcagctc gttggcagga tacaggtccc ggtatctgcc ggtgaatctg tgattctcga
      601 tcactggcac gattctcttg ccggcgatga aaggggtgcc cttggcgtca aactgtgtct
      661 cgttcttctc gaacacgata tcccatgcag gcataaagcc gggcaggccc ctctggaagg
      721 acagatttct gttcatctta aagtgcagga tgaagtcgcc ggttttcacg tcgtagtgca
      781 gaaagtcgaa gccctccagg aagtgcttgc ggctctcgtg attcttgatg gttttccaca
      841 cgaaggggtc cacgaagccg gtcaggggat cgatcttaga tgtatatggg gcaggcacgt
      901 aaaacaggaa gccagactgg gtgcccatct tggcaaagga ggtgaactgg tctgtcagct
      961 ggtatgggtt cagcacgcct cccactttct ctgctggata gtccttcagc accaggcaat
     1021 tcagcttatc gatcagcatc ttctcgaact gctggtacac ggccttctcg gcga
//
