LOCUS       sequence-126415-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS013
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      532..618
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      667..761
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tcgagcggat gtaccccgag gacggcgcgc tgaagggcga gatcaagcag aggctgaagc
       61 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      121 tgcaactgcc cggcgcgtac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      181 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      241 acgagctgta caagtaataa tatccgggta ggcgcaatca ctttcgtctc tactccgtta
      301 caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg aaagcacagc
      361 ggctggctga ggagataaat aataaacgag gggctgtatg cacaaagcat cttctgttga
      421 gttaagaacg agtatcgaga tggcacatag ccttgctcaa attggaatca ggtttgtgcc
      481 aataccagta gaaacagacg aaggatccca tggtacgcgt gctagaggca tcaaataaaa
      541 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      601 ctcctgagta ggacaaatcc gccgccctag acctaggctg tcagaccaag tttacgagct
      661 cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
      721 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagcact agggacagta
      781 agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc agtctgaatg
      841 acctgtcacg ggataatccg aagtggtcag actggaaaaa tcagagggca ggaactgctg
      901 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
      961 gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa aagg
//
