LOCUS       sequence-126427-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_omega2R
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     166..185
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     379..401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     393..410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     394..410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    411..467
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(480..496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(480..496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(528..534,535..552,553..558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    573..594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctataagctc ttttctctat gttacccgcc aagatatcct gtcaagcttg gtctcnagcg
       61 tagtgagacg cacagcattg ctctgtaanc ggatgccggn agcngaccag cccgatcagg
      121 gctcgtcaac ggntgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
      181 ttgtactgag agtgcnccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
      241 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggcgc
      301 aggcctcttc nctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc
      421 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg
      481 tcatagctgt ttcctgtgtg aaattgttat cnnctcacna ttccncacaa catacgagcc
      541 ggaagcatat agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgcg tctcacgctt
      661 gactgagacc aagcttgnta gtgnnnanac cacaatatat cctgccacca gncagccaac
      721 anctaccang accggngagc tcggcacaaa atctccactc gatacaagna gcccatcagt
      781 cc
//
