LOCUS       sequence-126429-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_omega2R
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    510..534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    622..643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(658..663,664..681,682..688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    696..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     701..723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     720..736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     720..736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    749..805
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(806..823)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(806..822)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(815..837)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggttgctctc gtttccggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
       61 atttctgtcc tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa
      121 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
      181 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
      241 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
      301 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
      361 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
      421 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
      481 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataaca
      541 agcttggtct cagtcaagcg tgagacgcag ctggcacgac aggtttcccg actggaaagc
      601 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      661 acactatatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      721 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
      781 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      901 atacgaagag gcctgcgccg atcgccc
//
