LOCUS       sequence-126447-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG
  TITLE     Orai1 is an essential pore subunit of the CRAC channel.
  JOURNAL   Nature. 2006 Sep 14. 443(7108):230-3.
  PUBMED    16921383
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Sep 14. 443(7108):230-3."
COMMENT     Sequence Label: Orai1 CFP
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..354
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     291..312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    480..601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(517..536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     571..590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(745..766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
       61 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      121 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      181 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      241 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      301 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
      361 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      421 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      601 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
      661 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      781 ga
//
