LOCUS       sequence-126454-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG
  TITLE     Orai1 is an essential pore subunit of the CRAC channel.
  JOURNAL   Nature. 2006 Sep 14. 443(7108):230-3.
  PUBMED    16921383
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Sep 14. 443(7108):230-3."
COMMENT     Sequence Label: Orai1 CFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(100..612,613..615,616..618))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     293..312
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     552..573
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      615..624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
       61 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      121 gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat
      181 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      241 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      301 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      361 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca
      421 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      481 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      541 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      601 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
      661 cagaattcgg gcatagtggc tgccgggcgt cagggggtgg tcccctctgt ggtccagctg
      721 gtcctgtaag cgggcaaact ccgccagctc gttgagctcc tggaactgtc ggtcggtctt
      781 atggctaacc agtgagcggt agaagtggac ggcgaa
//
