LOCUS       sequence-126462-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentivirus expressing iRFP670, Cre, and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pTRE:iRFP670-EFS:Cre-2A-GFP
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(288..341)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
       61 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
      121 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      181 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      241 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgaattctgg gccgggattt
      301 tcctccacgt ccccgcatgt tagaagactt cccctgccct cgccggagcc ggccggccca
      361 tcgccatctt ccagcaggcg caccattgcc cctgtttcac tatccaggtt acggatatag
      421 ttcatgacaa tatttacatt ggtccagcca ccagcttgca tgatctccgg tattgaaact
      481 ccagcgcggg ccatatctcg cgcggctccg acacgggcac tgtgtccaga ccaggccagg
      541 tatctctgac cagagtcatc cttagcgccg taaatcaatc ggtgagttgc ttcaaaaatc
      601 ccttccaggg cgcgagttga tagctggctg gtggcagatg gcgcggcaac accatttttt
      661 ctgacccggc aaaacaggta gttattcgga tcatcagcta caccagagac ggaaatccat
      721 cgctcgacca gtttagttac ccccaggcta agtgccttct ctacacctgc ggtgctaacc
      781 agcgttttcg ttctgccaat atggattaac attctcccac cgtcagtacg tgagatatct
      841 ttaaccctga tcctggcaat ttcggctata cgtaacaggg tgttataagc aatccccaga
      901 aatgccagat tacgtatatc ctggcagcga tcgctatttt ccatgagtga acgaacctgg
      961 tcgaaatcag t
//
