LOCUS       sequence-126465-       11492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  provides avaiM, eco47iiM, and ecot22iM that encode specific 
            methylases to enable transfer of plasmids from E. coli to Anabaena 
            sp. strain PCC 7120 and other strains. lacks a mobilization 
            function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
  TITLE     Reduction of conjugal transfer efficiency by three restriction 
            activities of Anabaena sp. strain PCC 7120.
  JOURNAL   J Bacteriol. 1997 Mar;179(6):1998-2005.
  PUBMED    9068647
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "1997-03"; volume: "179(6)"; pages: "1998-2005"
COMMENT     Sequence Label: pRL1124
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     214..233
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7858..7877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8096..8200)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8268..8286
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(9056..9871)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMHNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9779..9798
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9863..9882
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(10332..10349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10467..11010)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(10546..10565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtcgactc tagagtcgac gctctccctt atgcgactcc tgcattagga agcagcccag
       61 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
      121 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      181 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      241 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgaact
      301 gaacacgtcg cgtgaccgtg aaaacacctt cgaaccgcag ttgattaaga agcaccagac
      361 gcgcattacg cagatggaca gccagatttt atccctctac gccaaagata tgactacccg
      421 cgaaattgtc gccaccttca aagagatgta cgacgcagat gtgtcaccca cgctgatatg
      481 taaagtcact gatgcggtca aagagcaggt tgcagaatgg caaaatcgac ctctggatgc
      541 actgtatccc attgtttacc ttgactgtat cgtggtgaaa gttcgtcaag gtggcaccgt
      601 aattaacaaa gcggtgttcc ttgcactcgg tattaatact ggaggccaga aagagttgct
      661 gggcatgtgg ctggccgaga acgaaggggc gaagttctgg ctgggggggc tgacagagct
      721 taagaatcgg gggcttcagg atatccttat tgcctgcatg gacggtctga aaggctttcc
      781 ggatgcgata aacagcgtgt atccgtagac gcacatccag ttgtgcatca ttcatatggt
      841 gcgcaacagc ctgaaatacg tatcgtggaa ggactacaaa gccgtcactg gcgggttaaa
      901 aacggtgtat caggcgccaa cagaagaggc ggcgctagtg gcaccggagc agttcccgaa
      961 tgtctgggac gataaatacc cgcaaatcag caaaagctgg cgtgcgcact gggaaaatat
     1021 caacacgttc ttcgctattc gcccgatatc cgcaaggcca tctacaccac gaacgctatc
     1081 gaatcgctga acagcgtgat ccgtgcggcc attaagaagc gtaaagtgtt cccgactgac
     1141 gactcgctgc ggaaggtcat ttctctggcg atcaaggatg catcaaaaaa atggaatatg
     1201 ccaatccaga gctggcggct ggcgatgagt cgctttatta tcgagttcgg tgaccgcctg
     1261 agcgatcacc tttaatatgg cagcaattac acagaattat ttacagggtc tatcttttat
     1321 ttatcttatc taaaagatgt accacagatt tgccaacagc aaatgacaga ttaacaggaa
     1381 cagcgtttcc tatttgctta tatttcgctg acattgggcc ttcaaatacc cattcatcgg
     1441 ggaaggtctg tattcttgca tactcacgca cagttaatgg tcttgtttct tctgggtggc
     1501 aacgctctgt ttgtttctgt gctggggcgc atgttaatgt taggcttggt tcatcccatg
     1561 acaaacgacg agccatacca gttttgcccc cacctaagta aaaactcttg agcatgtatt
     1621 ctttttgaat atcttcagga agatctctcc agtagccacc gggaggaacc atacttagga
     1681 tctctgcttt tcttttggga tatttttgtc cttcagattc tggcacgtcg ctatcataca
     1741 gctctccctt ttttaatgca tctttaaggg ttaatatttt attgtaagaa gaaggccact
     1801 cataatcgat gccatcagca agatcatttc ttacagctac aatgatcaaa cgctcgcgtt
     1861 tttgcggcac tttgtagaaa atagccttaa gcactcttgg ctcaaataaa gtgtagccca
     1921 agtctgtgat aatatttttt attgtttcta aagttcgtcc agcatcatga tttagcaacc
     1981 ctcgaacatt ctctgctaaa agaactttcg gattgatttc tttagcggct cgggcgaatt
     2041 caaagaaaag ggtgccccgt gtatcttcaa aaccaagttt tttgcctgca taagagaatg
     2101 cctggcaagg aaagccacca gccagcacat caacggtatt cctataaggg gtgaagtcta
     2161 cttggctcac atcaccttca accacattcc attcaggcct attttttcgt aacgtcttac
     2221 aagcatggga gtcaatttca tttagtaaaa cagattttaa accggctttt tctaaaccta
     2281 aagccatccc ccctgcgcca gcaaataact ctaatacagt ataaacatta ttgcttattt
     2341 tagtctcatc aggccactgg cttttaaata aaaactgagc ttgttcaaaa tttttaagtt
     2401 gctctttttt ataaaatcga tagttgtttt cgtcatttct ttgagaaact aattttccag
     2461 cagtatccca acgccttaaa gtttcttttg aaacgcccaa aatgtctgca acttctgaaa
     2521 gtgaaaactc ttcctttaac atatttagta accatgccaa cattagtcat ggttactact
     2581 gtatgggttt acatgtgctt tgtcaaacat tgatcatggt tatcagccgt aagcgagccc
     2641 aaaatactta ataaaactat ctttatcagg gatggaaaaa ccttgctgcc caagacgttc
     2701 gctcaaaata tactcaattg cgtgagggag agcactatgg agttcttgca gagcatctgg
     2761 tctgccagtg acaagctcat agaaactcgc accatcaatg attctaatgt tagggttcga
     2821 aggacacaga gtaccactac ctttattgga aggagtaaag gggctgttat atctgatagg
     2881 tttacgaggg attatattaa caaagtacgc acagtaatcc ttaaatcggc tatgtttcgg
     2941 tgataccaac tcatctaagc ctttatattt atctgctaaa tccccgccag taaccgttga
     3001 gtatttattt ttcacctcag cgatgattct tctttcttcg ttgagaagat ccacaggacg
     3061 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     3121 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     3181 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     3241 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     3301 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     3361 tagcaattta actgtgataa actaccgcat taaagcttct agaggatccc ccctttcaat
     3421 ttggcttact cggatcttta atgctcttgc ctgctcaaca aatggtgttg cttttcgatt
     3481 tctttctaaa gctaaagcaa caaatcctgc tcggacttct tgatatggag tcacaagatc
     3541 atcattgctt tgaatatgat attgatatgg cactagggtt ttctccaaac atacacacat
     3601 ttgcgaagtg tctttcttcc atgtgtcccc atttgttggc tactgttacc tttgccagta
     3661 ggtaagacaa gaattttctc cacattaaaa ccaatttctt ctgcaatatt agaaagaatt
     3721 aaatcaaccg aaatatcaat acctgcatag cgaacattat catttaccat aaataaaggt
     3781 gagccatttt ttaaaactct aaagcattct ataataacgc aagccatttc atagaaatat
     3841 ccttttatca tacgaggaat accgttatta ttaagttttt ttctttcaag ctctcgctca
     3901 agaaagcgca agatactttg taacaattct tgtttatcaa gtatgcgtaa tgcttcctgc
     3961 caattgtgaa taagagactt ttctttgttt tcaacagtac aactcagcat atcttgccta
     4021 agttgtacta tatctctttc tcccacacct aatagagcta attctagagc gtatgtacgt
     4081 gtatagtcat aacgattaca atagggtgga gaggtaatga tacagtcgta aaaatcttgc
     4141 tcaaattcag gcagaatttt aagacatgaa gcattaaata aattaattcc acttctttga
     4201 gtcccataac aaaataatgt attacttatt aagtcaaagg aatcattcaa aattaatttt
     4261 atttgctcag taattgcttc atcgaaatcc agaattttac ctttatcaaa tttatctgat
     4321 ccacttttcc taaatgctct tttatcccaa cgtagatact gtccatcttt acgggtatag
     4381 ctgatagatt caagaataca caatagagca aaacggagaa cttgtttcac aagaatattc
     4441 tctttttcta tagaaaataa aaatctctct attgatgctt ctgtttcagg aggataagct
     4501 ccgtcagtaa ttcttaaacg attaagatat tttctattat tatgctgatt ccaaggtttt
     4561 tgtttgtacc attcaatcaa tctcaaaaaa tcttctcgac ggaattgtcg ctggattatt
     4621 ttccttactt caataatctc ttgaccaata ggtaacactt ctataccatc tgcttgaata
     4681 ccggcatcac tacaggcaaa tagcattgtt cctgctcctg cgaaggggtc aagaatcctc
     4741 tcaaatgatt ttttctcgta ctcgaatatt aactgattta ccaaatcaac tgaaaaagct
     4801 tctttatatt taaaccagcg atactgaggt tttgacttat tagcttgaaa actaactatt
     4861 tttcgactta aactttcatg caatcgcatc ttatctctaa aacgttgctc aagtgcttta
     4921 ttgaatcgag aaatttcttg tacaaaggaa tcactctctt gatcaagatc agtcgggtca
     4981 gtctttattt ctattggact ctcaagctca aatgaagtca ttttatattt ttcttgtaac
     5041 aaagaggtaa cttaagattc tatcaggaca aagcactatc gtctatcact taagatttga
     5101 accctatttt ttatagacta attgacaaca gttagaacct gattatactt tgttatactt
     5161 ctacagtaat caagttaaat attgaatagg gtaccgagct cgaattcatc ggtagggaga
     5221 ttttttcaag taacagaggt tttggatttt aaaaaatatt tccttgattt cgataaaacg
     5281 aataagttcc ctatgagctt tgttgttaaa actgagcttg agccagaaga ggttatggac
     5341 aagataaaga ttaactctga aaaaagcatg gaaaaggaat tggtgcagaa atatctgatt
     5401 ttgtttgaag aaatttttac attacagacg ctcagaagta tcctgggaat gatatctgac
     5461 tctcttgatg agcttgaaga gctaaagaga gtcataatcg aaaattttaa attagagtat
     5521 ggtcttcttg attaaataat aaatcattgt ttaatgggtt agagatgttg tcaatatcat
     5581 ctctaatgtt ttttgatttg ttttttgtct tccttttttt tactttctcc aattcttttg
     5641 gggtataatc tgaaggtata cccaatcttc ttatgcccat ttttacatac tcttcgttta
     5701 tttctatgcc tataaatctc ctgtctagag acttggcaac atctcctgtt gtaaagctac
     5761 cagaaaaagg gtctaagact acatcgcctg ggttcgatga agctagaata attctcctca
     5821 aaagggacgt cggtttctgt gtggggtggt tttcatattc ctccataaga aatcttactc
     5881 taggaaaatc ccaaacgttt cctggtactt tttgtgtgct ataaggttgg ggtgggtttt
     5941 ttctataatc aatcaaagcc cttttagatc ctgtttttgc ttcaaccaaa atatctttgc
     6001 tgttaaatgt gtattttttt tgatctttta ccatcattaa gattggttcg tacatggagc
     6061 caaagtgttt tttggcttga acacctgagc tgtcataaga ccaaactaat cgacttttta
     6121 tatggaagat ttttctacat tttatgtcaa tgtatggcat attctcagtg ctgttcataa
     6181 tatacatagt gccatgcttt tttagcactc tatggcattc atcgacgcat tcgaataacc
     6241 aatgaaggaa ttcaccctca tcccatgact caacaagtcc gtcaaagtcc ttaccaatat
     6301 tatatggggg gtctgcaaaa attaaatcaa cactctctgc tggcaatttc tttaattcag
     6361 tcaacgcatc gccatggata attttatttt tttcatcttt aaaatatcta gcttcaaaat
     6421 ctattttcat tttctacctc ttgtgtgact catagtctac tatgttaccg atatctagat
     6481 ctcgagctat caggctagag attttaccgc caaatcgaac cttattagag cggtttaggc
     6541 tggaccggca gttaaaattg gggcttgagc ggtaaacgag tgagggaatt tcaggtaaga
     6601 tacttcggat gaggagcaaa aaggtggttt atacttccta tacccaaaaa ggtggtttat
     6661 acttcctata ccccggttat ccggtggttt tctgtttgcg gaataaaaca gattagtgac
     6721 agttttctca gaatggcgtg ttccggtgac ttctgttttc gtgtgctgca tccggaagtg
     6781 tcaggcccgc cggatatgaa aaaggcctcc ggagagtacc gtgactttat tttcggcaca
     6841 aatacagggg tcgatggata aatacggcga tagtttcctg acggatgatc cgtatgtacc
     6901 ggcggaagac aagctgcaaa cctgtcagat ggagattgat ttaatggcgg atgtgctgag
     6961 agcaccgccc cgtgaatccg cagaactgat ccgctatgtg tttgcggatg attggccgga
     7021 ataaataaag ccgggcttaa tacagattaa gcccgtatag ggtattatta ctgaatacca
     7081 aacagcttac ggaggacgga atgttaccca ttgagacaac cagactgcct tctgattatt
     7141 aatatttttc actattaatc agaaggaata accatgaatt ttacccggat tgacctgaat
     7201 acctggaatc gcagggaaca ctttgccctt tatcgtcagc agattaaatg cggattcagc
     7261 ctgaccacca aactcgatat taccgctttg cgtaccgcac tggcggagac aggttataag
     7321 ttttatccgc tgatgattta cctgatctcc cgggctgtta atcagtttcc ggagttccgg
     7381 atggcactga aagacaatga acttatttac tgggaccagt cagacccggt ctttactgtc
     7441 tttcataaag aaaccgaaac attctctgca ctgtcctgcc gttattttcc ggatctcagt
     7501 gagtttatgg caggttataa tgcggtaacg gcagaatatc agcatgatac cagattgttt
     7561 ccgcagggaa atttaccgga gaatcacctg aatatatcat cattaccgtg ggtgagtttt
     7621 gacggattta acctgaacat caccggaaat gatgattatt ttgccccggt ttttacgatg
     7681 gcaaagtttc agcaggaagg tgaccgcgta ttattacctg tttctgtaca ggttcatcat
     7741 gcagtctgtg atggctttca tgcagcacgg tttattaata cacttcagac tcaaccaagt
     7801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8281 cgaggccctt tcgtcttcaa gaattttata aaccgtggag cgggcaatac tgagctgatg
     8341 agcaatttcc gttgcaccag tgcccttctg atgaagcgtc agcacgacgt tcctgtccac
     8401 ggtacgcctg cggccaaatt tgattccttt cagctttgct tcctgtcggc cctcattcgt
     8461 gcgctctagg atcctccggc gttcagcctg tgccacagcc gacaggatgg tgaccaccat
     8521 ttgccccata tcaccgtcgg tactgatccc gtcgtcaata aaccgaaccg ctacaccctg
     8581 agcatcaaac tcttttatca gttggatcat gtcggcggtg tcgcggccaa gacggtcgag
     8641 cttcttcacc agaatgacat caccttcctc caccttcatc ctcagcaaat ccagcccttc
     8701 ccgatctgtt gaactgccgg atgccttgtc ggtaaagatg cggttagctt ttacccctgc
     8761 atctttgagc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga
     8821 atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag
     8881 gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga
     8941 agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt
     9001 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
     9061 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     9121 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     9181 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     9241 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     9301 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     9361 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt atgcattcgt gattgcgcct
     9421 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     9481 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     9541 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     9601 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     9661 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     9721 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     9781 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     9841 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     9901 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     9961 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
    10021 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
    10081 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
    10141 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
    10201 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
    10261 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
    10321 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
    10381 atggcttacg aaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga
    10441 agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac
    10501 gaaatctgac gctcaaatca gtggtggcga acccgacagg actataaaga taccaggcgt
    10561 ttcccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
    10621 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
    10681 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
    10741 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
    10801 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
    10861 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
    10921 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
    10981 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaaggg gtctgacgct
    11041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11461 aatagtttgc gcaacgttgt tgccattgct gc
//
