LOCUS       sequence-12647-e        1529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: GFP-UtrCH
FEATURES             Location/Qualifiers
     source          1..1529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(12..14,15..17,18..728)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12..14,15..17,18..728)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(57..78)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(318..337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     665..686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatccaaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
       61 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      121 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      181 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
      241 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      301 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      361 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      421 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      481 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      541 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      601 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
      661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      721 tgtacaagtc cggaaccatg gccaagtatg gagaacatga agccagtcct gacaatgggc
      781 agaacgaatt cagtgatatc attaagtcca gatctgatga acacaatgac gtacagaaga
      841 aaacctttac caaatggata aatgctcgat tttcaaagag tgggaaacca cccatcaatg
      901 atatgttcac agacctcaaa gatggaagga agctattgga tcttctagaa ggcctcacag
      961 gaacatcact gccaaaggaa cgtggttcca caagggtaca tgccttaaat aacgtcaaca
     1021 gagtgctgca ggttttacat cagaacaatg tggaattagt gaatataggg ggaactgaca
     1081 ttgtggatgg aaatcacaaa ctgactttgg ggttactttg gagcatcatt ttgcactggc
     1141 aggtgaaaga tgtcatgaag gatgtcatgt cggacctgca gcagacgaac agtgagaaga
     1201 tcctgctcag ctgggtgcgt cagaccacca ggccctacag ccaagtcaac gtcctcaact
     1261 tcaccaccag ctggacagat ggactcgcct ttaatgctgt cctccaccga cataaacctg
     1321 atctcttcag ctgggataaa gttgtcaaaa tgtcaccaat tgagagactt gaacatgcct
     1381 tcagcaaggc tcaaacttat ttgggaattg aaaagctgtt agatcctgaa gatgttgccg
     1441 ttcggcttcc tgacaagaaa tccataatta tgtatttaac atctttgttt gaggtgctac
     1501 ctcagcaagt caccatagac taactcgag
//
