LOCUS       sequence-126481-        7687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch
            E
  TITLE     Paired cloning vectors for complementation of mutations in the 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.
  PUBMED    17639350
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2007-12"; volume: "188(6)"; pages: "551-63. Epub
            2007 Jul 17"
COMMENT     Sequence Label: pRL2697
FEATURES             Location/Qualifiers
     source          1..7687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(107..125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4512..4614
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4615..5274
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4681..4700)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(6281..6300)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6520..6542)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6628..6768
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6683..6702
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6783..6800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6954..7542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7034..7053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctaggcctt aagatctgat catatgcatc cgcgggcccg ggttacgaga tcgatgaatt
       61 cgagctcggt acccctagag tcgacctgca ggcatgcaag cttctcccta tagtgagtcg
      121 tattagcggc cgcaagccca agcgttttgt tattgggctg actcaagatg ataatgtcaa
      181 gaccttaggt atcgagggtg aaggcggtgt ccgtaagtgt tttaggatgt tacgcctggg
      241 taagtttgca gttagccacg ccaagtcctt aaaagacctt gcccttgtgg agtggttaaa
      301 gtcggggaaa tatcgctgca tggtggatga tatgccgtgt cagttaccag atgtaagcag
      361 ttacaaaatg gttatgcccc ggttacagtc tgtaaccagt tttcagccac tccaaacggc
      421 tcaaccgacc tcagaacaag cattacagcc cgtttcatcc ctcccagaac caagagatga
      481 aaccctagca aaagtggtaa aagcctgtct ggaagcgggt ttttcagaaa gtaaggtgat
      541 taaagaaatt ttgggctatc aaggcgggcg ttaccaagaa ggtaaatcgc tactggaaaa
      601 acttaaggat ttgtagcggt gcggtttagc attgactgtt acttgctgta caaggataat
      661 tttctcaaag cggaatggtg ttaaacccga ttattagagt cgtcatagca aaagcttgaa
      721 aactggcaag ggtgtactgg ttttcgttct ttttggttct aatggttatt ttttaccagt
      781 ttatttagta ataaccgagt gccaagtttc ttgttttaca tagcaaagtt agcaaaaaac
      841 attttgccgt gaatttccat ctgtaaaaag cgtcacggcg aaattcagta tttagtaaat
      901 acaaaaataa gtcaaacgtt gatcatgcaa cactttggcg tacaattgat tgaaaatttt
      961 gccgataggc aaagtttaaa ttacctatta aaaaaagttc agtgcttggg ggttgttatg
     1021 ctacttaacc gaagctaaat aaaacacaca tacacagaat tgttaccaca ccaaacacca
     1081 aacagaaaaa acgtgctttg aatttatcaa ttaatgggtg tagttaacaa gttttattta
     1141 gcttcaggtt aagcaaataa cattcaatag gtactggact gagacatttc caaatgatca
     1201 gcccagaaag cttaaataca aatgtatcac taaccctgct tacaagggaa ctacaaactg
     1261 aaataattct tggcacttac acggtcaggg tacacactcg cattggtcga gaaccttgtg
     1321 cgcggttgtg gtatttgtgt cgagcgcttg ataaggatgg gtctggtcat ttaacattgc
     1381 cattgcctgt agtacagacg ttcttagatt gtagtgataa gtctgtttac cgttggttac
     1441 aggatggaaa aaaaatagga gcattccgcc gctacaaaat taaagcagga atgataaccg
     1501 tctacttggg cggtatgttt caagtttgtt acaacctaaa tttaaaaaga tggggtgatg
     1561 ttgcagtagt gcctttagtc caagtgttaa gcgatttacg ctcactgacc actggcatag
     1621 tcacacaaag ctttcaacaa aagagccgct atgcggccaa cagacaactt aaaccagagt
     1681 acagaaaact ttttggagcg ccacacccga acgagctagt gaaggacaca aggcaatcct
     1741 ctcttaaatc gccagagggg gaagtacctt gtgtacttca cattagttcg tcgcggatat
     1801 tcgtctcaaa aagtttcatc cactatggta catctcaaaa agctgtcagc tgtgagttgg
     1861 gtattcacaa gcgcacagtg cgacgacatc aaaagcagct gggcatgaat cgccgtcaac
     1921 tttgccaagc caaaattgag tacaatcaac tccggcacgc tcggaataat gacgcgtctg
     1981 aattttgggc tttcactgga accaaaactg atatcggtta tcaagttatg ggtgacgcgg
     2041 ttgtgttttc tgatggcatt gcccctggag ccaaaaagag acagcccaac acttaccaaa
     2101 ttgacgcgac tgaatttgac ggtcgattat tcaaagtggg tgacaaggtg ttcatgaatc
     2161 ggtgcaacat ctaccgcgaa caattcaccc taacaacgat gtcagcagca cggcggaagt
     2221 accatttcaa actgtcacag tgccacttca gcgaaaatcg agccggtcgg gtaggcaacc
     2281 gttttgtaat aggttgccac tctggcgaga tatgaaggac atttttaaga attttttttt
     2341 agcgtgaaat gctgcggcaa ccagtatagt gatcgacacg aactagtgca gcatgaccga
     2401 gagcctatag ggtcgcggct ttttggcaat agagatcgta tgcagatcat gcgtagtact
     2461 ttcttcgacc ttaaccccaa agaaagctta ttagatggag aaggtcacaa ttagaaaata
     2521 ttcatcgtcc cgattgaccc ttaaccttta atcggtttcc acttgtaaaa aattcggcga
     2581 gccattcttc ttaaccctca gagtcgaact tcatacaaga atatacaaac cctttgggtt
     2641 taccactaaa acaacggcga taaaaaccta ataggtttcc acagaaacgt ctaaggattt
     2701 aggcgtgttg ttttggggca atttgagaaa atattaaacc agtcacaagt taacttgtcg
     2761 aagaaatatt gcagcattct gcaacacagg gaaaggtata acgtttccaa agtccggaac
     2821 gtgtgcagag ttctagaacc gatggatact ttaaaagtat cctcaagtgt agagcgtgtg
     2881 tttaggctgc gtcttttttt agcacccata ggggtctaaa cagcgttggc gtacattaga
     2941 agccaatcac ggatacagct ttgcgtcgct gttcgggtgt aacttcaaga tagcgttgca
     3001 gtgtgccaag gtcattgtga ccggatatct cttgaatgtg tcgcaacggg ataccggcgc
     3061 tagacatttg gttgagggca gtacggcgga aactgtgggt actgatgcct tctagcccga
     3121 ttcttttggc tgcatcgcgc aagattttat ccgccgcgta tcgcgtgagc ctatcgctga
     3181 ctcccctcat gccagggaac agggttcccg gtttggggtg atagtcagcc atgagtgcgg
     3241 ctagtcctgg ctggatgtca accacgcggg ttttgagttt ccctttggtg gtagacttcc
     3301 taaaggttag tgtttcgcct ttaatgtccg ttgtttggag tgctagagct tcactaacgc
     3361 ggcaaccggt gaatagacag atgccaaaca aaacgcgatc gcgcggtgtg gtgaatccgt
     3421 cgctaaacag tcgcctgagt tcgtcggagg tgagtatttt ggctcgtccg ttgccgttaa
     3481 ccttcataac ccttacagat taagcattcc aatactctac acgttatctg cattttgtgg
     3541 aattggtggc tatatctatt gattcttcgt atgctttctg ttgaaagcca gtgctagttt
     3601 agccaagctt atcaatcgtt gcacggtaaa atcaggcttt cgacgtaagg acagaatttt
     3661 gataacttca gcttatgttg tctcctgaag aaagcaactc tttgataaac gcgtcgatgg
     3721 ctttcccggc ggctgactgc ctacccattt ttagcttatt gagggtgcga tcgcgggttg
     3781 cctccaattt gtctagattg tctatactat tgtctatatt ctttctgtct tgcccggtag
     3841 cttgtccttc tgcctgaact accgggcttt cgattttccc cgcagttcct ccagttgcga
     3901 ttgaagtttg tctagacgtg ttgctagatt atcttctata cgcttgtcta taaattcatc
     3961 tatattgtct agattattag ttgtgacttt atctagacgt tgttcaatat tgtctatacg
     4021 ctgttcgagg gatgtgtcta gatgggtggg agtgtgagaa gtgggagtat taaacctgtt
     4081 acctgccaaa taccaacgaa caaattctgt taacagtgct gaggcgttgg aactttcaga
     4141 actagcaagg tttttaaaag actcccattc ttcggagtca atacgaaaag tggctagagt
     4201 tttgtctgcc atgattagat aatgtctata cttgtataga tagcagtata aactattgtc
     4261 tatattagtg tctatataag tctagatatc gtccattccg acagcatcgc cagtcactat
     4321 ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgggtaccg agctagagtc
     4381 tttgttttga cgccattagc gtacatcgaa taaatacctg tgacggaaga tcacttcgca
     4441 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
     4501 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
     4561 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     4621 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     4681 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     4741 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     4801 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     4861 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     4921 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     4981 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     5041 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     5101 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     5161 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     5221 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
     5281 taaggcagtt attggtgccc ttaaacgcct ggtgctacgc ctgaataagt gataataagc
     5341 ggatgaatgg cagaaattcg atgaattcgc gtgctataat tatactaatt ttataaggag
     5401 gaaaaaatat gggcattttt agtatttttg taatcagcac agttcattat caaccaaaca
     5461 aaaaataagt ggttataatg aatcgttaat aagcaaaatt catataacca aattaaagag
     5521 ggttataatg aacgagaaaa atataaaaca cagtcaaaac tttattactt caaaacataa
     5581 tatagataaa ataatgacaa atataagatt aaatgaacat gataatatct ttgaaatcgg
     5641 ctcaggaaaa ggccatttta cccttgaatt agtaaagagg tgtaatttcg taactgccat
     5701 tgaaatagac cataaattat gcaaaactac agaaaataaa cttgttgatc acgataattt
     5761 ccaagtttta aacaaggata tattgcagtt taaatttcct aaaaaccaat cctataaaat
     5821 atatggtaat ataccttata acataagtac ggatataata cgcaaaattg tttttgatag
     5881 tatagctaat gagatttatt taatcgtgga atacgggttt gctaaaagat tattaaatac
     5941 aaaacgctca ttggcattac ttttaatggc agaagttgat atttctatat taagtatggt
     6001 tccaagagaa tattttcatc ctaaacctaa agtgaatagc tcacttatca gattaagtag
     6061 aaaaaaatca agaatatcac acaaagataa acaaaagtat aattatttcg ttatgaaatg
     6121 ggttaacaaa gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca
     6181 tgcaggaatt gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag
     6241 ctataaatta tttaataagt aagttaaggg atgcaggtcg cctatatcgc cgacatcacc
     6301 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     6361 gtggcaggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
     6421 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
     6481 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     6541 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     6601 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     6661 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6721 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     6781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7141 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7381 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7621 ttatcaaaaa ggatccctgc cggccggtcc ggcgcgcccg ggctcgagtg cacgtacgac
     7681 gtctaga
//
