LOCUS       sequence-126485-        7432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDU1-E. coli shuttle vector (Km-r) with extended polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch
            E
  TITLE     Paired cloning vectors for complementation of mutations in the 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.
  PUBMED    17639350
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2007-12"; volume: "188(6)"; pages: "551-63. Epub
            2007 Jul 17"
COMMENT     Sequence Label: pRL2960
FEATURES             Location/Qualifiers
     source          1..7432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(80..98)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4999..5793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5053..5072)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5663..5682
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5999..6018)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6238..6260)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6346..6486
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6401..6420
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6501..6518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6672..7260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6752..6771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atccgcgggc ccgggttacg agatcgatga attcgagctc ggtaccccta gagtcgacct
       61 gcaggcatgc aagcttctcc ctatagtgag tcgtattagc ggccgcaagc ccaagcgttt
      121 tgttattggg ctgactcaag atgataatgt caagacctta ggtatcgagg gtgaaggcgg
      181 tgtccgtaag tgttttagga tgttacgcct gggtaagttt gcagttagcc acgccaagtc
      241 cttaaaagac cttgcccttg tggagtggtt aaagtcgggg aaatatcgct gcatggtgga
      301 tgatatgccg tgtcagttac cagatgtaag cagttacaaa atggttatgc cccggttaca
      361 gtctgtaacc agttttcagc cactccaaac ggctcaaccg acctcagaac aagcattaca
      421 gcccgtttca tccctcccag aaccaagaga tgaaacccta gcaaaagtgg taaaagcctg
      481 tctggaagcg ggtttttcag aaagtaaggt gattaaagaa attttgggct atcaaggcgg
      541 gcgttaccaa gaaggtaaat cgctactgga aaaacttaag gatttgtagc ggtgcggttt
      601 agcattgact gttacttgct gtacaaggat aattttctca aagcggaatg gtgttaaacc
      661 cgattattag agtcgtcata gcaaaagctt gaaaactggc aagggtgtac tggttttcgt
      721 tctttttggt tctaatggtt attttttacc agtttattta gtaataaccg agtgccaagt
      781 ttcttgtttt acatagcaaa gttagcaaaa aacattttgc cgtgaatttc catctgtaaa
      841 aagcgtcacg gcgaaattca gtatttagta aatacaaaaa taagtcaaac gttgatcatg
      901 caacactttg gcgtacaatt gattgaaaat tttgccgata ggcaaagttt aaattaccta
      961 ttaaaaaaag ttcagtgctt gggggttgtt atgctactta accgaagcta aataaaacac
     1021 acatacacag aattgttacc acaccaaaca ccaaacagaa aaaacgtgct ttgaatttat
     1081 caattaatgg gtgtagttaa caagttttat ttagcttcag gttaagcaaa taacattcaa
     1141 taggtactgg actgagacat ttccaaatga tcagcccaga aagcttaaat acaaatgtat
     1201 cactaaccct gcttacaagg gaactacaaa ctgaaataat tcttggcact tacacggtca
     1261 gggtacacac tcgcattggt cgagaacctt gtgcgcggtt gtggtatttg tgtcgagcgc
     1321 ttgataagga tgggtctggt catttaacat tgccattgcc tgtagtacag acgttcttag
     1381 attgtagtga taagtctgtt taccgttggt tacaggatgg aaaaaaaata ggagcattcc
     1441 gccgctacaa aattaaagca ggaatgataa ccgtctactt gggcggtatg tttcaagttt
     1501 gttacaacct aaatttaaaa agatggggtg atgttgcagt agtgccttta gtccaagtgt
     1561 taagcgattt acgctcactg accactggca tagtcacaca aagctttcaa caaaagagcc
     1621 gctatgcggc caacagacaa cttaaaccag agtacagaaa actttttgga gcgccacacc
     1681 cgaacgagct agtgaaggac acaaggcaat cctctcttaa atcgccagag ggggaagtac
     1741 cttgtgtact tcacattagt tcgtcgcgga tattcgtctc aaaaagtttc atccactatg
     1801 gtacatctca aaaagctgtc agctgtgagt tgggtattca caagcgcaca gtgcgacgac
     1861 atcaaaagca gctgggcatg aatcgccgtc aactttgcca agccaaaatt gagtacaatc
     1921 aactccggca cgctcggaat aatgacgcgt ctgaattttg ggctttcact ggaaccaaaa
     1981 ctgatatcgg ttatcaagtt atgggtgacg cggttgtgtt ttctgatggc attgcccctg
     2041 gagccaaaaa gagacagccc aacacttacc aaattgacgc gactgaattt gacggtcgat
     2101 tattcaaagt gggtgacaag gtgttcatga atcggtgcaa catctaccgc gaacaattca
     2161 ccctaacaac gatgtcagca gcacggcgga agtaccattt caaactgtca cagtgccact
     2221 tcagcgaaaa tcgagccggt cgggtaggca accgttttgt aataggttgc cactctggcg
     2281 agatatgaag gacattttta agaatttttt tttagcgtga aatgctgcgg caaccagtat
     2341 agtgatcgac acgaactagt gcagcatgac cgagagccta tagggtcgcg gctttttggc
     2401 aatagagatc gtatgcagat catgcgtagt actttcttcg accttaaccc caaagaaagc
     2461 ttattagatg gagaaggtca caattagaaa atattcatcg tcccgattga cccttaacct
     2521 ttaatcggtt tccacttgta aaaaattcgg cgagccattc ttcttaaccc tcagagtcga
     2581 acttcataca agaatataca aaccctttgg gtttaccact aaaacaacgg cgataaaaac
     2641 ctaataggtt tccacagaaa cgtctaagga tttaggcgtg ttgttttggg gcaatttgag
     2701 aaaatattaa accagtcaca agttaacttg tcgaagaaat attgcagcat tctgcaacac
     2761 agggaaaggt ataacgtttc caaagtccgg aacgtgtgca gagttctaga accgatggat
     2821 actttaaaag tatcctcaag tgtagagcgt gtgtttaggc tgcgtctttt tttagcaccc
     2881 ataggggtct aaacagcgtt ggcgtacatt agaagccaat cacggataca gctttgcgtc
     2941 gctgttcggg tgtaacttca agatagcgtt gcagtgtgcc aaggtcattg tgaccggata
     3001 tctcttgaat gtgtcgcaac gggataccgg cgctagacat ttggttgagg gcagtacggc
     3061 ggaaactgtg ggtactgatg ccttctagcc cgattctttt ggctgcatcg cgcaagattt
     3121 tatccgccgc gtatcgcgtg agcctatcgc tgactcccct catgccaggg aacagggttc
     3181 ccggtttggg gtgatagtca gccatgagtg cggctagtcc tggctggatg tcaaccacgc
     3241 gggttttgag tttccctttg gtggtagact tcctaaaggt tagtgtttcg cctttaatgt
     3301 ccgttgtttg gagtgctaga gcttcactaa cgcggcaacc ggtgaataga cagatgccaa
     3361 acaaaacgcg atcgcgcggt gtggtgaatc cgtcgctaaa cagtcgcctg agttcgtcgg
     3421 aggtgagtat tttggctcgt ccgttgccgt taaccttcat aacccttaca gattaagcat
     3481 tccaatactc tacacgttat ctgcattttg tggaattggt ggctatatct attgattctt
     3541 cgtatgcttt ctgttgaaag ccagtgctag tttagccaag cttatcaatc gttgcacggt
     3601 aaaatcaggc tttcgacgta aggacagaat tttgataact tcagcttatg ttgtctcctg
     3661 aagaaagcaa ctctttgata aacgcgtcga tggctttccc ggcggctgac tgcctaccca
     3721 tttttagctt attgagggtg cgatcgcggg ttgcctccaa tttgtctaga ttgtctatac
     3781 tattgtctat attctttctg tcttgcccgg tagcttgtcc ttctgcctga actaccgggc
     3841 tttcgatttt ccccgcagtt cctccagttg cgattgaagt ttgtctagac gtgttgctag
     3901 attatcttct atacgcttgt ctataaattc atctatattg tctagattat tagttgtgac
     3961 tttatctaga cgttgttcaa tattgtctat acgctgttcg agggatgtgt ctagatgggt
     4021 gggagtgtga gaagtgggag tattaaacct gttacctgcc aaataccaac gaacaaattc
     4081 tgttaacagt gctgaggcgt tggaactttc agaactagca aggtttttaa aagactccca
     4141 ttcttcggag tcaatacgaa aagtggctag agttttgtct gccatgatta gataatgtct
     4201 atacttgtat agatagcagt ataaactatt gtctatatta gtgtctatat aagtctagat
     4261 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     4321 atgcaatttc tatgcgcagt gggcgcatca cgctaaaaca ggggaatatc acgctcaacg
     4381 cggtgctggc cgaggagatt aacccgccca agggtgagac cccgttgaaa tggttgttgc
     4441 tgaccggcga accggtcgag tcgctagccc aagccttgcg cgtcatcgac atttataccc
     4501 atcgctggcg gatcgaggag ttccataagg catggaaaac cggagcagga gccgagaggc
     4561 aacgcatgga ggagccggat aatctggagc ggatggtctc gatcctctcg tttgttgcgg
     4621 tcaggctgtt acagctcaga gaaagcttca cgctgccgca agcactcagg gcgcaagggc
     4681 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     4741 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     4801 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     4861 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     4921 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     4981 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5041 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5101 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5161 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5221 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5281 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5341 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5401 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5461 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5521 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5581 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5641 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5701 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5761 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5821 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5881 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5941 tcatgctgga gttcttcgcc caccggcatc accggcgcca caggtgcggt tgctggcgcc
     6001 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     6061 tgtttcggcg tgggtatggt ggcaggcccg ctttatcaga agccagacat taacgcttct
     6121 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
     6181 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6241 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6301 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     6361 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     6421 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6481 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6841 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     6901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7081 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7321 gggattttgg tcatgagatt atcaaaaagg atccctgccg gccggtccgg cgcgcccggg
     7381 ctcgagtgca cgtacgacgt ctagacctag gccttaagat ctgatcatat gc
//
