LOCUS       sequence-126493-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and a C16orf80 targeting synthetic 
            single-guide RNA (sgRNA) element from U6 promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: lentiCRISPR - C16orf80 sgRNA 1
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        218..293
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    341..458
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gttagagagt attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
       61 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      121 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      181 gaaaggacga aacaccgtgt ctgagaagta aacccgtgtt ttagagctag aaatagcaag
      241 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
      301 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      361 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      421 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      481 gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca
      541 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      601 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
//
