LOCUS       sequence-126516-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pBAD-rxRFP1.2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     837..856
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga gctcgagcga gcttagttgc
      121 gctataggtc ggctgagctc acccgtggtt tccgagcgga tgtaccccga ggacggcgcc
      181 ctgaagagcg agatcaagaa ggggctgagg ctgaaggacg gcggccacta cgccgccgag
      241 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac
      301 atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtgcgaacgc
      361 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggagg tacaggcggg
      421 agtctggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      481 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      541 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
      601 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacattaag
      661 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag
      721 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag
      781 gacggcgtat tcatctacaa ggtgaagctg cgcggcacca acttcccccc cgacggcccc
      841 gtaatgcaga agaagaccat gggctgggag gctacgcgtg accaaaggtg cagtagtgtg
      901 gcttaagctt
//
