LOCUS       sequence-126518-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C9orf114 in mammalian cells with GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-C9orf114-gfp
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctctagactg ccggatctag ctagttaatt aagccaccat ggcggagcgc ggcaggaagc
       61 ggccgtgcgg cccgggtgaa cacggccaaa ggattgagtg gcgaaaatgg aagcaacaga
      121 agaaagagga gaaaaaaaaa tggaaggatc tcaagctgat gaaaaaactg gagcggcagc
      181 gggcacagga ggaacaggca aagcgcctgg aagaggagga ggcagcggca gagaaggagg
      241 accgcgggcg gccctacaca ctgagcgtag ccctgccggg ctccatcctg gacaatgctc
      301 agtcgccgga gcttcgcacc tacttggccg gtcagattgc cagagcctgt gccatcttct
      361 gtgtggatga gatcgtggtg tttgatgagg agggccagga tgccaagact gtggaggggg
      421 aattcacagg agttgggaag aaggggcagg cgtgcgtaca gctggcccgg atcctgcagt
      481 acctggagtg tccacagtac ctgaggaagg cgttcttccc caagcaccag gatctacagt
      541 ttgcagggct cctgaacccc ctggacagcc cccaccacat gcgtcaggat gaggaatccg
      601 agttccgaga gggcatcgtg gtggatcggc ccacccggcc aggccacggc tcctttgtca
      661 actgtggcat gaaaaaggag gtgaagattg acaagaacct ggagcccggg cttcgggtga
      721 ctgtgcgact gaaccagcag cagcacccag actgcaagac ctaccatggc aaagtggtat
      781 catcgcagga ccctcgcacc
//
