LOCUS       sequence-126519-       14337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Dajee M, Tarutani M, Deng H, Cai T, Khavari PA
  TITLE     Epidermal Ras blockade demonstrates spatially localized Ras 
            promotion of proliferation and inhibition of differentiation
  JOURNAL   Oncogene. 2002 Feb 28;21(10):1527-38
  PUBMED    11896581
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene.";
            date: "2002-02-28"; volume: "21(10)"; pages: "1527-3"
COMMENT     Sequence Label: LZRS-BRaf-ERTm
FEATURES             Location/Qualifiers
     source          1..14337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    893..1027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(917..936)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     971..990
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1257..1856)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1340..1360)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1837..1856
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1939..2438)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1987..2006)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2380..2403
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      4470..6259
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(6753..7544,7545..7613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7376..7395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7614..7718)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8255..8724
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8789..9146
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9211..9627
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9557..9579
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9595..9611
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    9637..10011
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     9949..9965
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    10041..10065
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10096..10125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(13412..13436)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             13749..14218
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgaat tagatccaga
      901 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      961 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1021 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     1081 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     1141 gtcggtgggc ctcgggggcg ggtgcggggt cggcggggcc gccccgggtg gcttcggtcg
     1201 gagccatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag
     1261 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     1321 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     1381 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     1441 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     1501 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     1561 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     1621 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     1681 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     1741 gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     1801 gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggaa
     1861 ggtcgtctcc ttgtgagggg tcaggggcgt gggtcagggg atggtggcgg caccggtcgt
     1921 ggcggccgac ctgcaggtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga
     1981 cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc
     2041 gcccctgagc ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca
     2101 gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg
     2161 ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt
     2221 cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg
     2281 tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg
     2341 tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca
     2401 tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattaat tctcatgttt
     2461 gacagcttat catcgataag ctgatcctca caggccgcac ccagcttttc ttccgttgcc
     2521 ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg agaatcccca
     2581 tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga ggctcaggac
     2641 gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg tgatgaggac
     2701 gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag cgaccatgaa
     2761 ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct gtattcactg
     2821 agcgtcgtct aataaagatg tctattgatc tcttttagtg tgaatcatgt ctgacgaggg
     2881 gccaggtaca ggacctggaa atggcctagg agagaaggga gacacatctg gaccagaagg
     2941 ctccggcggc agtggacctc aaagaagagg gggtgataac catggacgag gacggggaag
     3001 aggacgagga cgaggaggcg gaagaccagg agccccgggc ggctcaggat cagggccaag
     3061 acatagagat ggtgtccgga gaccccaaaa acgtccaagt tgcattggct gcaaagggac
     3121 ccacggtgga acaggagcag gagcaggagc gggaggggca ggagcaggag gggcaggagc
     3181 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg
     3241 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg
     3301 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg
     3361 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc
     3421 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga
     3481 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc
     3541 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca
     3601 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg
     3661 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca
     3721 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag
     3781 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct
     3841 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc
     3901 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga
     3961 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat
     4021 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat
     4081 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg
     4141 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat
     4201 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg
     4261 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt
     4321 gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc gcgttggaaa
     4381 acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc ctggcctcct
     4441 taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga aaattcacgc
     4501 ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta ctgggtatca
     4561 tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat taggatagca
     4621 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     4681 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
     4741 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
     4801 tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg
     4861 ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg atagcatatg
     4921 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
     4981 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
     5041 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     5101 ctacccagat atagattagg atagcatatg ctatccagat atttgggtag tatatgctac
     5161 ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg accaacaacc
     5221 ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc agcaatcgcg
     5281 cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc attagtggtt
     5341 ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca agttattaca
     5401 cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg tgcccccact
     5461 ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca ttggcgtgga
     5521 gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc tgtcggcgtc
     5581 cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg tcttggtccc
     5641 tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat gtgttgccca
     5701 tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc accccatgga
     5761 tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta ttgcccaagg
     5821 ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc cccgtccaaa
     5881 ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata caccttactg
     5941 ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag caggcgaaga
     6001 ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg actaaaatgg
     6061 ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc tcatggggtg
     6121 ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc atatgcttcc
     6181 cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac tactacccgg
     6241 gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta ttgctaatgc
     6301 cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg ttggtgtagc
     6361 ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg tgtaagagct
     6421 tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact gcaaagtctg
     6481 ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata aggatgtcaa
     6541 ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc
     6601 cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg
     6661 gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gatctggccg caaatcaatc
     6721 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6781 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6841 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6901 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6961 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7021 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7081 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7141 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7201 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7261 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7321 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7381 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7441 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7501 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7561 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7621 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7681 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7741 ccagctttgc tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga
     7801 cttgttctat gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat
     7861 gttaattcca ttttaaatgc acagatgttt ttatttcata agggtttcaa tgtgcatgaa
     7921 tgctgcaata ttcctgttac caaagctagt ataaataaaa atagataaac gtggaaatta
     7981 cttagagttt ctgtcattaa cgtttccttc ctcagttgac aacataaatg cgctgctgag
     8041 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
     8101 caattagttg atttttattt ttgacatata catgtgaaag accccacctg taggtttggc
     8161 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
     8221 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     8281 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     8341 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     8401 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     8461 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     8521 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     8581 cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     8641 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     8701 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     8761 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
     8821 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
     8881 tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg
     8941 ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc
     9001 gatcgttttg gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga
     9061 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga
     9121 agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg
     9181 tttctgtatt tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg
     9241 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
     9301 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
     9361 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
     9421 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
     9481 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
     9541 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
     9601 ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt atatggggca
     9661 cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct
     9721 ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg
     9781 gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac
     9841 acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac
     9901 acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac
     9961 gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatccc
    10021 agtgtggtgg tacgggaatc acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt
    10081 cagtcgacca ccatggagca gaagctgatt tccgaggagg acctgggatc cgcggcgctg
    10141 agcggtggcg gtggtggcgg cgcggagccg ggccaggctc tgttcaacgg ggacatggag
    10201 cccgaggccg gcgccggcgc cggcgccgcg gcctcttcgg ctgcggaccc tgccattccg
    10261 gaggaggtgt ggaatatcaa acaaatgatt aagttgacac aggaacatat agaggcccta
    10321 ttggacaaat ttggtgggga gcataatcca ccatcaatat atctggaggc ctatgaagaa
    10381 tacaccagca agctagatgc actccaacaa agagaacaac agttattgga atctctgggg
    10441 aacggaactg atttttctgt ttctagctct gcatcaatgg ataccgttac atcttcttcc
    10501 tcttctagcc tttcagtgct accttcatct ctttcagttt ttcaaaatcc cacagatgtg
    10561 gcacggagca accccaagtc accacaaaaa cctatcgtta gagtcttcct gcccaacaaa
    10621 cagaggacag tggtacctgc aaggtgtgga gttacagtcc gagacagtct aaagaaagca
    10681 ctgatgatga gaggtctaat cccagagtgc tgtgctgttt acagaattca ggatggagag
    10741 aagaaaccaa ttggttggga cactgatatt tcctggctta ctggagaaga attgcatgtg
    10801 gaagtgttgg agaatgttcc acttacaaca cacaactttg tacgaaaaac gtttttcacc
    10861 ttagcatttt gtgacttttg tcgaaagctg cttttccagg gtttccgctg tcaaacatgt
    10921 ggttataaat ttcaccagcg ttgtagtaca gaagttccac tgatgtgtgt taattatgac
    10981 caacttgatt tgctgtttgt ctccaagttc tttgaacacc acccaatacc acaggaagag
    11041 gcgtccttag cagagactgc cctaacatct ggatcatccc cttccgcacc cgcctcggac
    11101 tctattgggc cccaaattct caccagtccg tctccttcaa aatccattcc aattccacag
    11161 cccttccgac cagcagatga agatcatcga aatcaatttg ggcaacgaga ccgatcctca
    11221 tcagctccca atgtgcatat aaacacaata gaacctgtca atattgatga cttgattaga
    11281 gaccaaggat ttcgtggtga tggaggatca accacaggtt tgtctgctac cccccctgcc
    11341 tcattacctg gctcactaac taacgtgaaa gccttacaga aatctccagg acctcagcga
    11401 gaaaggaagt catcttcatc ctcagaagac aggaatcgaa tgaaaacact tggtagacgg
    11461 gactcgagtg atgattggga gattcctgat gggcagatta cagtgggaca aagaattgga
    11521 tctggatcat ttggaacagt ctacaaggga aagtggcatg gtgatgtggc agtgaaaatg
    11581 ttgaatgtga cagcacctac acctcagcag ttacaagcct tcaaaaatga agtaggagta
    11641 ctcaggaaaa cacgacatgt gaatatccta ctcttcatgg gctattccac aaagccacaa
    11701 ctggctattg ttacccagtg gtgtgagggc tccagcttgt atcaccatct ccatatcatt
    11761 gagaccaaat ttgagatgat caaacttata gatattgcac gacagactgc acagggcatg
    11821 gattacttac acgccaagtc aatcatccac agagacctca agagtaataa tatatttctt
    11881 catgaagacc tcacagtaaa aataggtgat tttggtctag ctacagagaa atctcgatgg
    11941 agtgggtccc atcagtttga acagttgtct ggatccattt tgtggatggc accagaagtc
    12001 atcagaatgc aagataaaaa tccatacagc tttcagtcag atgtatatgc atttgggatt
    12061 gttctgtatg aattgatgac tggacagtta ccttattcaa acatcaacaa cagggaccag
    12121 ataattttta tggtgggacg aggatacctg tctccagatc tcagtaaggt acggagtaac
    12181 tgtccaaaag ccatgaagag attaatggca gagtgcctca aaaagaaaag agatgagaga
    12241 ccactctttc cccaaattct cgcctctatt gagctgctgg cccgctcatt gccaaaaatt
    12301 caccgcagtg catcagaacc ctccttgaat cgggctggtt tccaaacaga ggattttagt
    12361 ctatatgctt gtgcttctcc aaaaacaccc atccaggcag ggggatatgg tgcgtttcct
    12421 gtccacaagc ttatgcgaaa tgaaatgggt gcttcaggag acatgagggc tgccaacctt
    12481 tggccaagcc ctcttgtgat taagcacact aagaagaata gccctgcctt gtccttgaca
    12541 gctgaccaga tggtcagtgc cttgttggat gctgaaccgc ccatgatcta ttctgaatat
    12601 gatccttcta gacccttcag tgaagcctca atgatgggct tattgaccaa cctagcagat
    12661 agggagctgg ttcatatgat caactgggca aagagagtgc caggctttgg ggacttgaat
    12721 ctccatgatc aggtccacct tctcgagtgt gcctggctgg agattctgat gattggtctc
    12781 gtctggcgct ccatggaaca cccggggaag ctcctgtttg ctcctaactt gctcctggac
    12841 aggaatcaag gtaaatgtgt ggaaggcatg gtggagatct ttgacatgtt gcttgctacg
    12901 tcaagtcggt tccgcatgat gaacctgcag ggtgaagagt ttgtgtgcct caaatccatc
    12961 attttgctta attccggagt gtacacgttt ctgtccagca ccttgaagtc tctggaagag
    13021 aaggaccaca tccaccgtgt cctggacaag atcacagaca ctttgatcca cctgatggcc
    13081 aaagctggcc tgactctgca gcagcagcat cgccgcctag ctcagctcct tctcattctt
    13141 tcccatatcc ggcatatgag taacaaacgc atggagcatc tctacaacat gaaatgcaag
    13201 aacgtggtac ccctctatga cctgctcctg gagatgttgg atgcccaccg ccttcatgcc
    13261 ccagccagtc gcatgggagt gcccccagag gagcccagcc agacccagct ggccaccacc
    13321 agctccactt cagcacattc cttacaaacc tactacatac ccccggaagc agagggcttc
    13381 cccaatgagc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggtgata
    13441 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
    13501 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctattttcga
    13561 gcggccgcca gcacagtggt cgacgataaa ataaaagatt ttatttagtc tccagaaaaa
    13621 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
    13681 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
    13741 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
    13801 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
    13861 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
    13921 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
    13981 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
    14041 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
    14101 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
    14161 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
    14221 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
    14281 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgct
//
