LOCUS       sequence-126524-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.1, nuclear 
            localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pNuc-rxRFP1.1
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..81
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(85..93,94..108)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     852..871
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             922..942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             946..966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagcttgccg ccaccatggg aggttctcat catcatcatc atcatggtat ggctagcatg
       61 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg
      121 agcgagctta gttgcgctat aggtcggctg agctcacccg tggtttccga gcggatgtac
      181 cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc
      241 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      301 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      361 gaacagtgcg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      421 ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      481 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      541 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      601 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      661 aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      721 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
      781 gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg caccaacttc
      841 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggctac gcgtgaccaa
      901 aggtgcaaaa gtgtggctga tccaaaaaag aagagaaagg tagacccgaa gaaaaaacga
      961 aaggtagatc caaagaagaa gagaaagtaa tctaga
//
