LOCUS       sequence-126525-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Redox-Sensitive Red Fluorescent Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Fan Y, Chen Z, Ai HW
  TITLE     Monitoring redox dynamics in living cells with a redox-sensitive red
            fluorescent protein.
  JOURNAL   Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 
            2015 Feb 20.
  PUBMED    25666702
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac5041988";
            journalName: "Anal Chem"; date: "2015-03-3- 3"; volume: "87"; issue:
            "5"; pages: "2802-10"
COMMENT     Sequence Label: pcDNA3-rxRFP
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..81
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(85..93,94..108)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     852..871
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aagcttgccg ccaccatggg aggttctcat catcatcatc atcatggtat ggctagcatg
       61 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg
      121 agcgagctta gttgcgctat aggtcggctg agctcacccg tggtttccga gcggatgtac
      181 cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc
      241 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      301 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      361 gaacagtgcg aacgcgccga gggccgccac tccaccggcg acatggacga gctgtacaag
      421 ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      481 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      541 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      601 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      661 aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      721 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttaaccag
      781 gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg caccaacttc
      841 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggctac gcgtgaccaa
      901 aggtgctgca gtgtggctta agctggaatt c
//
