LOCUS       sequence-126527-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mUCH37 (ISF2) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-mUCH37 (ISF2)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..100
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tatacatatg catcatcacc atcaccatgg
       61 taagcctatc cctaaccctc tcctcggtct cgattctacg gaaaacctgt attttcaggg
      121 aattgatccc ttcacccata tgtcgagcaa tgccggggag tggtgtctca tggaaagcga
      181 ccccggggtc ttcaccgagc tcattaaagg attcggttgc cgaggggccc aagtagaaga
      241 aatatggagc ttagaacctg agagttttga aaagctaaag ccagttcatg ggttaatttt
      301 tcttttcaag tggcagccag gagaagaacc tgctggctct gtggttcagg actccagact
      361 tgaaacaata ttttttgcca agcaggtaat taataatgct tgtgccactc aggctatcgt
      421 aagtgtacta ctgaactgta cgcatcaaga tgtgcattta ggagagacat tatcagaatt
      481 taaagaattt tcccaaagtt ttgatgcagc tatgaagggg ctggcattga gtaactcaga
      541 tgtgattcga caagtgcaca acagttttgc caggcagcag atgtttgaat ttgatacaaa
      601 gacaccagcc aaagaggagg atgcttttca ctttgtcagt tacgttccag tcaatgggag
      661 gctgtatgaa ctagatgggt taagagaagg cccaattgat ttaggtgcat gcaatcaaga
      721 tgactggatt actgcagtga ggccagtaat agagaaaagg attcaaaagt atagtgaagg
      781 agaaattcga tttaatttaa tggccattgt a
//
