LOCUS       sequence-126529-        1089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mUCH37 (ISF2) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-mUCH37 (ISF2)
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             22..63
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcatcatc accatcacca tggtaagcct atccctaacc ctctcctcgg tctcgattct
       61 acggaaaacc tgtattttca gggaattgat cccttcaccc atatgtcgag caatgccggg
      121 gagtggtgtc tcatggaaag cgaccccggg gtcttcaccg agctcattaa aggattcggt
      181 tgccgagggg cccaagtaga agaaatatgg agcttagaac ctgagagttt tgaaaagcta
      241 aagccagttc atgggttaat ttttcttttc aagtggcagc caggagaaga acctgctggc
      301 tctgtggttc aggactccag acttgaaaca atattttttg ccaagcaggt aattaataat
      361 gcttgtgcca ctcaggctat cgtaagtgta ctactgaact gtacgcatca agatgtgcat
      421 ttaggagaga cattatcaga atttaaagaa ttttcccaaa gttttgatgc agctatgaag
      481 gggctggcat tgagtaactc agatgtgatt cgacaagtgc acaacagttt tgccaggcag
      541 cagatgtttg aatttgatac aaagacacca gccaaagagg aggatgcttt tcactttgtc
      601 agttacgttc cagtcaatgg gaggctgtat gaactagatg ggttaagaga aggcccaatt
      661 gatttaggtg catgcaatca agatgactgg attactgcag tgaggccagt aatagagaaa
      721 aggattcaaa agtatagtga aggagaaatt cgatttaatt taatggccat tgtatctgac
      781 agaaaaatga tatatgaaca gaagatagca gagttacaaa gacaacttgc agaggaaccc
      841 atggatacag atcaaggtag taccgtgtta agtgctattc agtcagaagt tgccagaaac
      901 cagatgctta ttgaagaaga ggtacaaaag ttaaagagat ataagattga aaatatcaga
      961 aggaagcata attacttgcc tttcattatg gagttgttaa agaccttagc tgagcaccag
     1021 cagttaattc cacttgtgga aaaggcaaaa gaaaaacaga atgcaaagaa agctcaggaa
     1081 actaagtga
//
