LOCUS       sequence-12653-e        7532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Gu J, Kagawa S, Takakura M, Kyo S, Inoue M, Roth JA, Fang B
  TITLE     Tumor-specific transgene expression from the human telomerase 
            reverse transcriptase promoter enables targeting of the therapeutic 
            effects of the Bax gene to cancers.
  JOURNAL   Cancer Res. 2000 Oct 1. 60(19):5359-64.
  PUBMED    11034071
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2000 Oct 1. 60(19):5359-64."
COMMENT     Sequence Label: pAdv/TERT
FEATURES             Location/Qualifiers
     source          1..7532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..106
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     polyA_signal    493..717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     706..723
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             5086..5277
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5271..5293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5379..5519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5434..5453
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5534..5551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5705..6293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5785..5804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6464..7255,7256..7324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7087..7106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7325..7429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7497..7515
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttccatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtata cgggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc aagtaatatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tctgtgttac tcatagcgcg taatatttgt ctagggccgc
      361 ggggactttg accgtttacg tggagactcg cccaggtgtt tttctcaggt gttttccgcg
      421 ttccgggtca aagttggcgt tttattatta tagtcagctc cgtggggatc ccccctcgag
      481 ccccagctgg ttccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
      541 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
      601 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
      661 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
      721 aggctgatca gcgagctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
      781 agaattccag cacactggcg gccgttacta gtggatccga gctcggtacc aagcttactt
      841 agatcgcaga tctatggcgg gggtggccgg ggccagggct tcccacgtgc gcagcaggac
      901 gcagcgctgc ctgaaactcg cgccgcgagg agagggcggg gccgcggaaa ggaaggggag
      961 gggctgggag ggcccggagg gggctgggcc ggggacccgg gaggggtcgg gacggggcgg
     1021 ggtccgcgcg gaggaggcgg agctggaagg tgaaggggca ggacgggtgc ccgggtcccc
     1081 agtccctccg ccacgtgggg agcgcggtcc tgggcgtctg tgcccgcgaa tccactggga
     1141 gcccggcctg gccccgacag cgcagctgct ccgggcggac ccgggggtct gggccgcgct
     1201 tccccgcccg cgcgccgctc gcgctcccag ggtgcaggga cgccagcgag ggccccagcg
     1261 gagagaggtc gaatcggcct aggctgtggg gtaacccgag ggaggggcca acgcgtatat
     1321 ctggcccgta catcgcgaag cagtcgagtc gacatcgatg ctagagtgga aggtgctgag
     1381 gtacgatgag acccgcacca ggtgcagacc ctgcgagtgt ggcggtaaac atattaggaa
     1441 ccagcctgtg atgctggatg tgaccgagga gctgaggccc gatcacttgg tgctggcctg
     1501 cacccgcgct gagtttggct ctagcgatga agatacagat tgaggtactg aaatgtgtgg
     1561 gcgtggctta agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg
     1621 ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc
     1681 atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag
     1741 cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc
     1801 tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg
     1861 cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag cttcccgttc
     1921 atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga
     1981 acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc
     2041 ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg
     2101 gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga
     2161 ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact
     2221 ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag
     2281 agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg
     2341 gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt
     2401 gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga
     2461 ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag
     2521 aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa
     2581 tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat
     2641 aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac
     2701 gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag
     2761 ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat
     2821 ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa
     2881 gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg
     2941 cgacttaccg cagccggtgg gcccgtaaat cacacctatt accgggtgca actggtagtt
     3001 aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct
     3061 gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag
     3121 ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt
     3181 gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc
     3241 tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt
     3301 cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc
     3361 gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg
     3421 aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag
     3481 catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg
     3541 cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc
     3601 gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg
     3661 cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc
     3721 tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa
     3781 aggctgtccg tgtccccgta tacagacttg agaggcctgt cctcgaccga tgcccttgag
     3841 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3901 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     3961 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     4021 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     4081 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     4141 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     4201 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     4261 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt
     4321 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     4381 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     4441 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
     4501 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc
     4561 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca
     4621 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     4681 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     4741 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     4801 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     4861 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     4921 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     4981 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     5041 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     5101 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     5161 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     5221 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5281 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5341 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     5401 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     5461 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5521 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5581 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5641 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6121 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6301 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6361 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6421 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6481 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6541 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6601 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6661 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6721 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     6781 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6841 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6901 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6961 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7021 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7081 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7141 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7201 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7261 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7321 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7381 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7441 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     7501 ggcgtatcac gaggcccttt cgtcttcaag aa
//
