LOCUS       sequence-126530-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting the repetitive sequence of human MUC4 exon 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1661-sgMUC4-E3(F+E)
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(196..229)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     233..253
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        548..851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agatccgacg cgccatctct aggcccgcgc cggccccctc gcacggactt gtgggagaag
       61 ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc
      121 ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga
      181 taggcttgga tttctataac ttcgtatagc atacattata cgaagttata aacagcacaa
      241 aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataag tatcccttgg
      301 agaaccacct tgttggtggc gtgacctgtg gatgctggtt taagagctat gctggaaaca
      361 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      421 tgcttttttt ctcgagtact aggatccatt aggcggccgc gtggataacc gtattaccgc
      481 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      541 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      601 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      841 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      901 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      961 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
//
