LOCUS       sequence-126534-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector that expresses shRNA under the 
            mouse U6 promoter. A CMV-EGFP reporter cassette is included in the 
            vector to monitor expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja 
            J, Rooney DL, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs
            L
  TITLE     A lentivirus-based system to functionally silence genes in primary 
            mammalian cells, stem cells and transgenic mice by RNA interference.
  JOURNAL   Nat Genet. 2003 Mar . 33(3):401-6.
  PUBMED    12590264
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2003 Mar . 33(3):401-6."
COMMENT     Sequence Label: pLL3.7
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..406
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     325..345
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     422..438
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     423..439
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(481..514)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
       61 atccagtttg gttagtaccg ggcccgctct agagatccga cgcgccatct ctaggcccgc
      121 gccggccccc tcgcacagac ttgtgggaga agctcggcta ctcccctgcc ccggttaatt
      181 tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct
      241 gttcttttta atactagcta cattttacat gataggcttg gatttctata agagatacaa
      301 atactaaatt attattttaa aaaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
      361 ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgttaacg cgcggtgacc
      421 ctcgaggtcg acggtatcga taagctcgct tcacgagatt ccagcaggtc gagggaccta
      481 ataacttcgt atagcataca ttatacgaag ttatattaag ggttccaagc ttaagcggcc
      541 gcgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
      601 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      781 ttggcagtac atcaagtgta tcatatgcca
//
