LOCUS       sequence-126539-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Jahreiss L, Menzies FM, Rubinsztein DC
  TITLE     The itinerary of autophagosomes: from peripheral formation to 
            kiss-and-run fusion with lysosomes.
  JOURNAL   Traffic. 2008 Apr;9(4):574-87. Epub 2008 Jan 7.
  PUBMED    18182013
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic."; 
            date: "2008-04"; volume: "9(4)"; pages: "574-87. Epub 2008 Jan 7"
COMMENT     Sequence Label: mCherry-hLC3B-pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    96..320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     309..326
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        346..364
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     346..363
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(428..802)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
ORIGIN
        1 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc
       61 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc
      121 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
      181 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
      241 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
      301 ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagcattta ggtgacacta
      361 tagaataggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
      421 attcttacac tgacaatttc atcccgaacg tctcctggga ggcgtagacc atgtacagga
      481 atccatcttc atctttctca ctctcataca cctctgagat tggtgtggag acgctgacca
      541 tgctgtgtcc gttcaccaac aggaagaagg cctgattagc attgagctgt aagcgccttc
      601 taattatctt gatgagctca ctcatgttga catggtcagg tacaaggaac tttgttttat
      661 ccagaacagg aagctgcttc tcacccttgt atcgttctat tatcaccggg attttggttg
      721 gatgctgctc tcgaataagt cggacatctt ctactctttg ttcgaaggtg cggcgctgct
      781 tgaaggtctt ctccgacggc atggatcctt gcttgtacag ctcgtccatg ccgccggtgg
      841 agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
      901 tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct
//
