LOCUS       sequence-126544-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construct for expression of OmpT protease in the pET28a plasmid 
            using the restriction sites NcoI and EcoRI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Wu C, Tran JC, Zamdborg L, Durbin KR, Li M, Ahlf DR, Early BP, 
            Thomas PM, Sweedler JV, Kelleher NL
  TITLE     A protease for 'middle-down' proteomics.
  JOURNAL   Nat Methods. 2012 Jun 17;9(8):822-4. doi: 10.1038/nmeth.2074.
  PUBMED    22706673
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-06-17- 17"; volume: "9"; 
            issue: "8"; pages: "822-4"
COMMENT     Sequence Label: pET28-OmpT
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(66..113)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     109..127
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(180..197)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct
       61 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
      121 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag
      181 tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt cgacggagct cgaattctta
      241 aaatgtgtac ttaagaccag cagtagtgat gaagttatag ttttctatac ctgctccatt
      301 tttgctgtag tctgaagtgt tattattgtg atcataaagt gaagtattac cttttttatt
      361 cgtaacccga ttccatgcgc cttcaacata aacttttgcg ttaggtgtga cgtaataacc
      421 tgcattgact gcaacagaat agtaattttg gtctttgacc ttactgcgat aagtgattct
      481 ttttcccggg tcatagtgtt catcgttatc agatgattcc acccagccgc tgtatttaaa
      541 tgtgccaccg agttcaaaat cttcataacg ataacttcca gtcaagccaa tgtagggcat
      601 tttaaaacgt tgtttgtagc cgattgctct ttctccattc gggaaggagc cgatatcatc
      661 tctgaatccc tcctcagaac tgtagatata ggaaccacct ctggctgtaa agctataacg
      721 gctttcctga tatccggcca tgagtcccag gcggtaat
//
