LOCUS       sequence-126546-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of alpha-bungarotoxin mRNA that can be 
            injected into embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Swinburne IA, Mosaliganti KR, Green AA, Megason SG
  TITLE     Improved Long-Term Imaging of Embryos with Genetically Encoded 
            alpha-Bungarotoxin.
  JOURNAL   PLoS One. 2015 Aug 5;10(8):e0134005. doi: 
            10.1371/journal.pone.0134005. eCollection 2015.
  PUBMED    26244658
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-5"; volume: "10(8):e0134005. doi"; pages: " 
            10.1371/journal.pone.0134005. eCollection 2015"
COMMENT     Sequence Label: pmtb-t7-alpha-bungarotoxin
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(358..377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(359..377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(385..403)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(386..403)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccggttccgg atacgtatga attcttactg cagatcatcc agggcgctgc ttagggtgag
       61 ggttgcactt gtcggtgcta cagcaggtca cctcttcgta aggtttcttg ctagggcagg
      121 tagcggcgca tcccagctcg accaccttgc ctctgctgct acagaaggcg tcgcaccaca
      181 tctttctgta gcacaggttc tcgccaggag ggcaggtcac agcgctgata gggctggtgg
      241 cggtggtgtg gcacacgatg gtgtatccca ggtccaggca cacgattgtc accaccacca
      301 gggtcagcag cagggtcttc atggtggcgg atcctgcaaa aagaacaagt agcttctccc
      361 tatagtgagt cgtattagta ctcttctata gtgtcaccta aataggccta ggatggctga
      421 actgtaaaag aaagggaaac tgttagaatt gcactgctgc cagggagagg cgtttcagca
      481 agcatgtgac catctggagt caacttcctg ttttgctctg cattctacag tctgaccgct
      541 ggtgggcgct agtgacagcg cgcgcctcag atatgccaga caaaggaagt ccctctgcat
      601 tctctcatta ttaccataaa aggcaatggt ttgagccgct ttgcggctgc cggcggtgtg
      661 gcactcagcc agagtcgtag ggctcagtaa aaaggaacag atttcgacat tcatcacacc
      721 aggaccactc cctaaatttt acacaaatga agtttcacat cctcttccac cctcgcctgc
      781 gatcaattac acaaccgcat ttcaacgggt gtctgccgcc gggtgttact gcgtcacaga
      841 tctgcagtca gtccacggac tcctcgtgag cggagacttc acactaaagc atttctggac
      901 ggtcactctc agtcggttca gttcatagtc tagcctcaaa aatcaccgag atcctgaaaa
      961 actgatgggc tctgcttcga cagtcacagc ggctgaagtg cagccagaga actccgcagc
     1021 cgttattaaa gtggaccaca cctctaaaat tcaaatcttt catagcggat acaacacttc
//
