LOCUS       sequence-126547-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of alpha-bungarotoxin mRNA that can be 
            injected into embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Swinburne IA, Mosaliganti KR, Green AA, Megason SG
  TITLE     Improved Long-Term Imaging of Embryos with Genetically Encoded 
            alpha-Bungarotoxin.
  JOURNAL   PLoS One. 2015 Aug 5;10(8):e0134005. doi: 
            10.1371/journal.pone.0134005. eCollection 2015.
  PUBMED    26244658
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-5"; volume: "10(8):e0134005. doi"; pages: " 
            10.1371/journal.pone.0134005. eCollection 2015"
COMMENT     Sequence Label: pmtb-t7-alpha-bungarotoxin
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..42
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        24..42
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     79..95
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     79..95
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(129..140,141..328)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
       61 caccgcggtg gcggccgctc tagaactagt ggatctgctg ggcttgctga aggtaggggg
      121 tcaagaacca gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt
      181 atttttgggg atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact
      241 taattacatt tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca
      301 aaaagtactt attttttgga gatcacttgg gcccggctcg agggcctagt cattacctat
      361 actcaacgtt tactctgaga aacttccata ttttagaggt aaaatgctca gaattgtaaa
      421 aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat aaaagtcaca tcacctcatt
      481 gtccttggta tcagcaagca cttttaacac aatcagctgt ttttgtttat aactcccact
      541 gaaggccatg tgcattactc ccaagttaca cagtaaaaag tcacatgtag tttatcatga
      601 tcactgctgc tcaagcacag ctttatctgt tattcagtgt ggcaatatct gaaatgtgtg
      661 caaaatatta tttattgtag ctgatcacca acactttcag tgtttttttt tttctacttt
      721 taatgagaat tatgttctta ggatttatcc ttataaacct ctttaaaaag aaatgttatg
      781 tctgcataga cacgacatga aattatgaac agaaaattta actatttaag atcaagactt
      841 gacatataac atttctaatg aacattaaac ctgaggtagg cccacttgca aaaatgtaac
      901 tgctatcact cactttatac atcattgtgt aacttgtaca cacaaaaata gtgaagcaat
      961 gtggcatgga atgcaggtcc cattcattca aagttattaa agcactttct taaagtgcat
     1021 tactactgag ctaagaccag ctagttgctc atacaacata ctacaagtag tgtcatccta
     1081 atttggataa aaa
//
