LOCUS       sequence-126557-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Device: FACS Standard Color Controls - High RFP (first unit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: pJ02B2Rm:Gm_AF
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             32..40
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(115..149)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      complement(160..187)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(203..274)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaagttcttc tcctttacgc atcattagaa aacctcctta gcatgattaa gatgtttcag
       61 tacgaaaatt gctttcattg ttgatctcct ttttaagtga acttgggccc agtagctagc
      121 acagtaccta ggactgagct agctgtcaaa agctataaac gcagaaaggc ccacccgaag
      181 gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata aaacgaaagg
      241 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggacctttat taagcaccgg
      301 tggagtgacg accttcagca cgttcgtact gttcaacgat ggtgtagtcc tcgttgtggg
      361 aggtgatgtc cagtttgatg tcggttttgt aagcacccgg cagctgaacc ggttttttag
      421 ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca
      481 ttttgatttc acctttcaga gcaccatcct ccgggtacat acgttcggtg gaagcttccc
      541 aacccatggt ttttttctgc ataaccggac cgtcggacgg gaagttggta ccacgcagtt
      601 taactttgta gatgaactca ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac
      661 caccatcttc gaagttcata acacgttccc atttgaaacc ttccgggaag gacagtttca
      721 ggtagtccgg gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg
      781 gg
//
