LOCUS       sequence-126563-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standardized mini-Tn5 delivery plasmid, gentamicin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI
  TITLE     New transposon tools tailored for metabolic engineering of 
            gram-negative microbial cell factories.
  JOURNAL   Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 
            10.3389/fbioe.2014.00046. eCollection 2014.
  PUBMED    25389526
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2014-10-28"; volume: "2:46. doi"; pages:
            " 10.3389/fbioe.2014.00046. eCollection 2014"
COMMENT     Sequence Label: pBAMD1-6
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..47
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(194..727)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gatctatcaa caggagtcca agactagtct gtctcttata cacatcttca gagattttat
       61 gcttgtaaac cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtccgc
      121 aattaattag acaattgtcc aatttaccga acaactccgc ggccgggaag ccgatctcgg
      181 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
      241 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
      301 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
      361 caatgccctg cctccggtgc tctccggaga ctgcgagatc atagatatag atctcactac
      421 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
      481 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
      541 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
      601 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
      661 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
      721 gtaacatcgt tgctgctcca taacatc
//
