LOCUS       sequence-126566-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-K12
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..67)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 agtggcaggg cggggcgtaa acgccatggg catgtagtca aaagcctccg gtcggaggct
       61 tttgacttgg ctgaggagtg ccgttaatta agttcataat cccagcacca gttgtctttc
      121 catttgcggg tcaagcaacg tcacatgcag ccccaccaga cgcacaccgc gcccgccgcg
      181 gcgttcatcc caggttttac gcgcggtggc gattagatca gctttattca gccgcggcca
      241 gacgtgctcc tgggtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgagc
      301 aatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc
      361 gataatcgct tcacattcag accagtgatg aatatcttcc gccatcgtgc gttccacgcc
      421 gacggatttt cgcaaccgtt cgctgttaac atcgcgttcg tcaatcccct gactacgctc
      481 ccacaaaatg cggccaaatt tgccaaagcg tttaagcagc atcaccagat cacacttttg
      541 tacatcaccg caggtccgca gccccatcgc ttccagtttt gccgctgaga ctttgccgac
      601 gccggggatt tttgccagcg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat
      661 cacaaactgg ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg
      721 tgccacgccc gcagacgccg tcagttgca
//
