LOCUS       sequence-126568-         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-K13
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(17..48)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc ttttgacttg gctgaggagt
       61 gccgttaatt aagtttaaaa tagcccaagt tgcccggtca taagtgtagc aaaattatcc
      121 tcaataaaag ggagtattcc ctccgccacg ggttgtagct ggcgggtcag atagtgttcg
      181 taatccagtg gtgaacgttg gtagtccagc ggctccgggc cgttggtggt ccatacgtac
      241 ttaatggtgc cgcgattctg atattgcaag gggcgaccac gcttttggtt ttcttcatcg
      301 gcaaggcgag cggcgcgtac atgaggcggc acattacgct gatactcgct cagcggacgg
      361 cgaaggcgtt tacggtaaac cagtcgcgca tccagttcac ccgccatcag tttgtcgatg
      421 gtttcgcgta catattcctg atatggctcg ttgcggaaga tgcgcaggta tagctcctgc
      481 tgaaactgct gggccagcgg cgtccagtcg gtgcgcacgg tttccagccc tttaaacacc
      541 atccgctgct tgtcgccctc ctgaatcagt ccggcataac gctttttact gcc
//
