LOCUS       sequence-126569-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-L2
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(40..71)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gatgagtggc agggcggggc gtaaacgcca tgggcatgta gtcaaaagcc tccggtcgga
       61 ggcttttgac ttggctgagg agtgccgtta attaagttta aaatagccca agttgcccgg
      121 tcataagtgt agcaaaatta tcctcaataa aagggagtat tccctccgcc acgggttgta
      181 gctggcgggt cagatagtgt tcgtaatcca gtggtgaacg ttggtagtcc agcggctccg
      241 ggccgttggt ggtccatacg tacttaatgg tgccgcgatt ctgatattgc aaggggcgac
      301 cacgcttttg gttttcttca tcggcaaggc gagcggcgcg tacatgaggc ggcacattac
      361 gctgatactc gctcagcgga cggcgaaggc gtttacggta aaccagtcgc gcatccagtt
      421 cacccgccat cagtttgtcg atggtttcgc gtacatattc ctgatatggc tcgttgcgga
      481 agatgcgcag gtatagctcc tgctgaaact gctgggccag cggcgtccag tcggtgcgca
      541 cggtttccag ccctttaaac accatccgct gcttgtcgcc ctcctgaatc agtccggcat
      601 aacgcttttt actgccggta tcggctccgc gaatggttgg catcagaaaa cggcagaaat
      661 gggtttcata ctccagttct aatgcgctgg tcagccgttg tttttgcagc gtttccgccc
      721 accaggcgtt aacgtgctgc accagtgcac gaccgatt
//
