LOCUS       sequence-126580-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-R4
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
       61 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
      121 gtttttttgg acgcgtacaa ctcaagtctg acataaatga ccgctatgag cactgcaatt
      181 acacgccaga tcgttctcgc taccgcaacc accggtatga accagatcgg tgcgcactat
      241 gaaggccaca agatcattga gattggtgcc gttgaagtgg tgaaccgtcg cctgacgggc
      301 aacaacttcc atgtttatct caaacccgat cggctggtgg atccggaagc ctttggcgta
      361 catggtattg ccgatgaatt tttgctcgat aagcccacgt ttgccgaagt agccgatgag
      421 ttcatggact atattcgcgg cgcggagttg gtgatccata acgcagcgtt cgatatcggc
      481 tttatggact ac
//
