LOCUS       sequence-126583-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BiFC empty vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Gou JY, Felippes FF, Liu CJ, Weigel D, Wang JW
  TITLE     Negative regulation of anthocyanin biosynthesis in Arabidopsis by a 
            miR156-targeted SPL transcription factor.
  JOURNAL   Plant Cell. 2011 Apr;23(4):1512-22. doi: 10.1105/tpc.111.084525. 
            Epub 2011 Apr 12.
  PUBMED    21487097
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.084525"; journalName: "Plant Cell"; date: 
            "2011-04"; volume: "23"; issue: "4"; pages: "1512-22"
COMMENT     Sequence Label: JW771
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(69..252)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(105..284)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    532..553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(568..573,574..591,592..598)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    606..622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     611..633
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     630..646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggagaacctg cgtgcaatcc atcttgttca atccccatgg tcgatcgaca gatctgcgaa
       61 agctcgagtg cagattattt ggattgagag tgaatatgag actctaattg gataccgagg
      121 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
      181 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
      241 ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
      301 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
      361 catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgccacc atgttggcaa
      421 gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      481 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
      661 ggtccccaga ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag
      721 gcttacgcag caggtctcat caagacgatc tacccgagca ataatctcca ggaaatcaaa
      781 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
      841 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
      901 ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact
      961 gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     1021 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aa
//
