LOCUS       sequence-126587-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector hexahistidine tagged variant of the Thermotoga 
            maritima M-domain protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Kieft JS, Batey RT
  TITLE     A general method for rapid and nondenaturing purification of RNAs.
  JOURNAL   RNA. 2004 Jun;10(6):988-95.
  PUBMED    15146082
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-06"; volume: "10(6)"; pages: "988-95"
COMMENT     Sequence Label: pTmaM4
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..84
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(558..576)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      572..619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(794..799,800..816,817..822))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(849..867)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        935..1039
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggccatcac
       61 catcaccatc acatcgaggg aaggccggca gctgaaaacc tgtacttcca gggggacgtt
      121 ctttcactga tcgaaaaagt ggaaaaggaa ctcgatcagg aaaagatgaa gaaaagcgca
      181 gaaaagttcc tgaaggcaga gttcaccctg gaagatttca aagaacagct tcaggagatg
      241 aaaaaactcg gacctctttc ttccattctt gagatgcttc caggtgcacc gaaggtcgat
      301 gtggagatga gtgaaaagga gctgaaaaag atcgaggcca tcataaattc gatgaccata
      361 gaagagagaa gaaatcccgg tataataaac gccagtagaa aaaggagaat cgcacgtgga
      421 agtggaacta ccgtccagga tgtgaacaaa cttctcaaaa gctatgaaca gatgaaagct
      481 ctaatgaaga ggatgaaaaa ataatgagga tccggctgct aacaaagccc gaaaggaagc
      541 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      601 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc
      661 ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg
      721 acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt
      781 gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag
      841 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      901 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
//
