LOCUS       sequence-126592-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, Copeland TD, 
            Waugh DS
  TITLE     Tobacco etch virus protease: mechanism of autolysis and rational 
            design of stable mutants with wild-type catalytic proficiency.
  JOURNAL   Protein Eng. 2001 Dec . 14(12):993-1000.
  PUBMED    11809930
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng. 2001 Dec . 14(12):993-1000."
COMMENT     Sequence Label: pRK793
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..186
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     350..372
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     364..381
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     365..381
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
       61 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
      121 ctactgccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt
      181 atcaggctga aaatcttatc tcatccgcca aaacagccaa gctgccattc gccattcagg
      241 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      301 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      361 cgttgtaaaa cgacggccag tgccaagctt gcctgcaggt cgactctaga ggatccttat
      421 tagcgacggc gacgacgatt catgagttga gtcgcttcct taactggctg aaaaggctct
      481 tcaggtttca ccatgaaaac tttatggccc ccccacaata ctgagtcagc atttaatcgc
      541 caaccactaa cccactgctg cgcctcctga tttgtcaaca attccatgaa gtttttcggc
      601 acgcttgtga aataattgtt tgtgttggtg aaattcgatg ctgagtgtat accaacaatg
      661 aacccatctc tagttgatac taatggactg ccacactgcc catccttggt ttgaatccaa
      721 tgcttccaga atatgc
//
