LOCUS       sequence-126595-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For bacterial expression of recombinant human ch-TOG tagged with MBP
            and His..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle 
            SJ
  TITLE     The mesh is a network of microtubule connectors that stabilizes 
            individual kinetochore fibers of the mitotic spindle.
  JOURNAL   Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.
  PUBMED    26090906
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-06-19"; volume: "4. doi"; pages: " 10.7554/eLife.07635"
COMMENT     Sequence Label: MBP-ch-TOG-His6
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..56
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(835..852)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acaacaacaa taacaataac aacaacctcg ggctggaagt tctgttccag gggcccgaat
       61 tcgggatcct aatgggagat gacagtgagt ggttgaaact gccagttgat cagaaatgtg
      121 aacacaagct gtggaaagca aggttaagtg ggtatgaaga ggccctgaag atcttccaga
      181 aaataaagga tgaaaagagc ccagagtggt ccaaattttt aggattgatc aaaaaatttg
      241 tcactgattc caatgcagtg gttcaattga aaggattaga agctgcactt gtttatgttg
      301 aaaatgccca tgtagcagga aaaaccacag gagaagttgt gtcaggtgtt gtaagtaagg
      361 tgttcaatca acctaaagct aaagccaagg agctgggcat agagatctgt cttatgtaca
      421 tagagattga gaaaggagag gctgttcaag aagagctcct gaaaggcttg gacaataaga
      481 atcccaagat catagtggcc tgtatagaga cactgaggaa agccttaagt gaatttggtt
      541 ccaaaatcat cttgcttaag ccaattatca aagtgttgcc aaaactcttt gagtctcgag
      601 agaaggctgt tcgagatgaa gccaaactaa ttgctgtgga gatttacaga tggattcggg
      661 atgctctgag acccccatta caaaatataa actctgttca gttgaaagaa ctagaagaag
      721 aatgggtcaa actgccaaca agtgctccta gacctactcg atttcttcgt tcccaacaag
      781 aactagaagc taaattggaa caacaacagt ctgctggtgg agatgctgaa ggaggtggtg
      841 atgatggtga tgaggtgcca ca
//
