LOCUS       sequence-126614-        4537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Citrine reporter for transfer fxns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)
FEATURES             Location/Qualifiers
     source          1..4537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..963
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1059..1130
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1146..1173
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1704..2654)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3416..3510)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3541..4332,4333..4401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHATRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4164..4183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgaa gacaaccacg cattgctgtt cgattcgtta ccaattgaca
      121 attgattgga cgttcaatat aatgctagct actagagaag cgggcccaag ttcacttaaa
      181 aaggagatca acaatgaaag caattttcgt actgaaacat cttaatcatg ctggggaggg
      241 tttctaatgt ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta
      301 gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaagg tgatgctact
      361 tacggtaaat tgaccttaaa atttatttgt actactggta aattgccagt tccatggcca
      421 accttagtca ctactttagg ttatggtttg atgtgttttg ctagataccc agatcatatg
      481 aaacaacatg actttttcaa gtctgccatg ccagaaggtt atgttcaaga aagaactatt
      541 tttttcaaag atgacggtaa ctacaagacc agagctgaag tcaagtttga aggtgatacc
      601 ttagttaata gaatcgaatt aaaaggtatt gattttaaag aagatggtaa cattttaggt
      661 cacaaattgg aatacaacta taactctcac aatgtttaca tcatggctga caaacaaaag
      721 aatggtatca aagttaactt caaaattaga cacaacattg aagatggttc tgttcaatta
      781 gctgaccatt atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt accagacaac
      841 cattacttat cctatcaatc tagattatcc aaagatccaa acgaaaagag agatcacatg
      901 gtcttgttag aatttgttac tgctgctggt attacccatg gtatggatga attgtacaaa
      961 tgatgaactc aaagcccgcc gaaaggcggg cttttctgtc cttgagagtc gggcattgtc
     1021 ttctaaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
     1081 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1141 acactggctc accttcgggt gggcctttct gcgtttatac ctagggtacg ggttttgctg
     1201 cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca
     1261 gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg
     1321 gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt
     1381 tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat
     1441 gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat
     1501 ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa
     1561 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct
     1621 ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga
     1681 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt
     1741 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc
     1801 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg
     1861 tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt
     1921 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta
     1981 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat
     2041 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc
     2101 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta
     2161 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa
     2221 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat
     2281 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg
     2341 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc
     2401 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca
     2461 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt
     2521 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc
     2581 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca
     2641 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga
     2701 tgggctagtc aatgataatt actagtcctt ttcccgggtg atctgggtat ctgtaaattc
     2761 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga
     2821 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa
     2881 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt
     2941 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa
     3001 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt
     3061 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct
     3121 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc
     3181 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc
     3241 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt
     3301 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca
     3361 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct
     3421 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     3481 aggtcattac tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc
     3541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3661 cagtgctgca atgataccgc gtgacccacg ctcaccggct ccagatttat cagcaataaa
     3721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3901 cagctccggt tcccaacgat caaggcgagt tgcatgatcc cccatgttgt gcaaaaaagc
     3961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4141 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     4201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4381 acggaaatgt tgaatactca tgcgaaacga tcctcatcct gtctcttgat cagatcatga
     4441 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     4501 cccaacctta ccagagggcg ccccagctgg caattcc
//
