LOCUS       sequence-126617-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Citrine reporter for transfer fxns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             270..986
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1082..1153
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1169..1196
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1727..2677)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3439..3533)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3564..4355,4356..4424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHATRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4187..4206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgaa gacaaccacg cattgctgtt cctgcaggtc gaggataaat
      121 atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gcatgtacta
      181 aagcgggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa
      241 catcttaatc atgctgggga gggtttctaa tgtctaaagg tgaagaatta ttcactggtg
      301 ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg
      361 gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg
      421 gtaaattgcc agttccatgg ccaaccttag tcactacttt aggttatggt ttgatgtgtt
      481 ttgctagata cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag
      541 gttatgttca agaaagaact atttttttca aagatgacgg taactacaag accagagctg
      601 aagtcaagtt tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta
      661 aagaagatgg taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt
      721 acatcatggc tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca
      781 ttgaagatgg ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg
      841 gtccagtctt gttaccagac aaccattact tatcctatca atctagatta tccaaagatc
      901 caaacgaaaa gagagatcac atggtcttgt tagaatttgt tactgctgct ggtattaccc
      961 atggtatgga tgaattgtac aaatgatgaa ctcaaagccc gccgaaaggc gggcttttct
     1021 gtccttgaga gtcgggcatt gtcttctaag gatccaaact cgagtaagga tctccaggca
     1081 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1141 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1201 tacctagggt acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta
     1261 tcagaatcgc agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa
     1321 ttgccatgat tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga
     1381 ttcgataagc agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag
     1441 ttgtctcagg tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct
     1501 attaggtgtt acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt
     1561 gaatgcacca aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc
     1621 tgtgcatatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
     1681 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
     1741 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
     1801 cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
     1861 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat
     1921 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
     1981 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
     2041 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
     2101 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
     2161 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
     2221 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
     2281 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
     2341 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
     2401 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
     2461 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
     2521 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
     2581 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     2641 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     2701 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttcccgg
     2761 gtgatctggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     2821 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     2881 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     2941 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     3001 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     3061 gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt
     3121 cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac
     3181 aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg
     3241 ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt
     3301 cagcagttcc tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc
     3361 attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt
     3421 actgtcccta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     3481 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     3541 gctctcgaac cccagagtcc cgcttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgtgaccc acgctcaccg
     3721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3841 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttgcatga
     3961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     4201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatgcgaaa cgatcctcat
     4441 cctgtctctt gatcagatca tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     4501 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
//
