LOCUS       sequence-126637-        3204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid for building 3n2, 5n1, 5n2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(977..994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1148..1736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1228..1247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1907..2698,2699..2767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHATRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2530..2549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2768..2872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2940..2958
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcattgctgt tctcagtgat agagattgac atccctatca gtgatagata taatgagcac
       61 tactagagaa gcgggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
      121 tactgaaaca tcttaatcat gctggggagg gtttctaatg gcacgtaccc cgagccgtag
      181 cagcattggt agcctgcgta gtccgcatac ccataaagca attctgacca gcaccattga
      241 aatcctgaaa gaatgtggtt atagcggtct gagcattgaa agcgttgcac gtcgtgccgg
      301 tgcaagcaaa ccgaccattt atcgttggtg gaccaataaa gcagcactga ttgccgaagt
      361 gtatgaaaat gaaagcgaac aggtgcgtaa atttccggat ctgggtagct ttaaagccga
      421 tctggatttt ctgctgcgta atctgtggaa agtttggcgt gaaaccattt gtggtgaagc
      481 atttcgttgt gttattgcag aagcacagct ggaccctgca accctgaccc agctgaaaga
      541 tcagtttatg gaacgtcgtc gtgagatgcc gaaaaaactg gttgaaaatg ccattagcaa
      601 tggtgaactg ccgaaagata ccaatcgtga actgctgctg gatatgattt ttggtttttg
      661 ttggtatcgc ctgctgaccg aacagctgac cgttgaacag gatattgaag aatttacctt
      721 cctgctgatt aatggtgttt gtccgggtac acagcgtgca gcaaacgacg aaaactacgc
      781 tttagcagct taatgaactt ccggcaatta aaaaagcggc taaccacgcc gcttttttta
      841 cgtctgcacc ttgagagtcg ctcgttgtct tcgctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtgttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1561 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2041 taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2221 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2281 aacgatcaag gcgagttgca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2341 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2401 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2461 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2521 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2581 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2641 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2701 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2761 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2821 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2881 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2941 ataggcgtat cacgaggccc tttcgtgttc aagaattctg gcgaatcctc tgaccagcca
     3001 gaaaacgacc tttctgtggt gaaaccggat gctgcaattc agagcgccag caagtggggg
     3061 acagcagatg acctgaccgc cgcagagtgg atgtttgaca tggtgatgac tatcgcacca
     3121 tcagccagaa aaccgaattt tgctgggtgg gctaacgata tccgcctgat gcgtgaacgt
     3181 gacggacgta acgaagacaa atgt
//
