LOCUS       sequence-126668-        5708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal-HA-tagged mouse Tropomodulin3, WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pCMV5-HA-mTmod3-WT
FEATURES             Location/Qualifiers
     source          1..5708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     853..873
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             934..960
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2038..2660
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2237..2256)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2682..2702)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2687..3017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2868..3003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3055..3074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3056..3074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3089..3105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3089..3105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3102..3124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3113..3129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3137..3143,3144..3161,3162..3167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3182..3203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3320..3337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3490..4078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3570..3589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4249..5040,5041..5109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4872..4891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5110..5214)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5241..5669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5328..5347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5538..5559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgatc tatacattga
      181 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
      241 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      301 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      541 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      721 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
      841 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      901 ctcgtttagt gaaccgtcag aattgccatg gcatacccat acgacgtgcc agactacgct
      961 ggaattcaga tggcactgcc gttccggaag gacttggggg actacaagga cctggatgag
     1021 gacgagctcc ttggcaagct gtccgaatca gagctgaaac agctggagac tgtcctggat
     1081 gatcttgacc ccgagaatgc ccttctgcct gcagggtttc ggcagaagaa ccagacatca
     1141 aagtctgcca cgggaccctt tgatagagaa cggctccttt cttacctaga gaagcaagca
     1201 ttggagcaca aagaccggga cgattatgtg ccctacactg gagagaagaa agggaaaatt
     1261 tttatcccca aacagaagcc agcgcagact cttacagaag aaactatatc cctcgaccca
     1321 gagttagaag aagctttgac aagtgcttca gacacagagc tgtgcgacct tgcagctatt
     1381 cttgggatgc acaatctgat tgcagacaca ccgttctgtg atgtgctggg aagtagtaac
     1441 ggtgtcaacc aagagcgttt tcccaatgtg gttaaagggg aaaagattct tccagtgttt
     1501 gatgagccac caaatccaac caacgttgaa gagagtttaa agagaattag ggaaaacgat
     1561 gcccgtcttg ttgaagttaa tttgaataat ataaaaaata tcccaattcc aactctaaaa
     1621 gattttgcaa agactttgga agccaacacg catgtgaaac atttcagtct cgcggccacc
     1681 aggagcaatg accccgtggc tgttgctttt gcagatatgc tgaaagtgaa caaaactttg
     1741 aagagcttaa atatggagtc caacttcatc acaggcgcag gggttttagc actgattgat
     1801 gctttgagag ataatgagac cctgatggag ctcaagattg acaatcagag acagcagttg
     1861 ggcacctcag tagaattaga aatggccaag atgcttgagg aaaatacaaa tatccttaaa
     1921 tttggatatc agttcacgca gcagggaccc cgaaccaggg cagccaacgc gataacgaag
     1981 aacaatgact tagtgcgcaa gaggcgaatt gaaggagacc accagtaacg ggatcccggg
     2041 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     2101 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     2161 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     2221 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     2281 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     2341 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     2401 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     2461 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     2521 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tgccatggcc
     2581 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     2641 cagtagatgc ctgttgaatt gggtacgcgg ccagcttctg tggaatgtgt gtcagttagg
     2701 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2761 gtcagcaacc aggtgtggaa aagtccccag gctccccagc aggcagaagt atgcaaagca
     2821 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2881 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2941 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3001 gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aattccctat
     3061 agtgagtcgt attaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3121 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3181 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aggccgcgtt
     3481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3661 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     3721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3901 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5221 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5281 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5341 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
     5401 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5461 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5521 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5581 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5641 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacaaaat attaacgttt
     5701 acaatttc
//
