LOCUS       sequence-126675-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for kanamycin resistance gene plant expression 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB Tnos:NptII:Pnos (GB0226)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..37
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        137..316
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(404..423)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tacagcgact tagtttaccc gccaatatat cctgtcagga tccgtctcat gacggagata
       61 catgagaatt aagggagtca cggttatgac ccccgccgat gacgcgggac aagccgtttt
      121 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag
      181 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
      241 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
      301 cctcggtatc caattagagt ctcatattca ctctcaactc gatcgaggca tgattgaaca
      361 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
      421 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
      481 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
      541 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
      601 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
      661 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
      721 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacaccgca tcgagcgagc
      781 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
      841 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgccc
//
