LOCUS       sequence-126676-        7141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of human XRCC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pET28-hXRCC1
FEATURES             Location/Qualifiers
     source          1..7141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4..81)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     287..306
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     390..409
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        390..408
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    409..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             448..470
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2391..2408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2461..2479)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2475..2522
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2559..3014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2646..2665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2856..2877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3107..3922)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3107..3922)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3830..3849
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3914..3933
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4044..4632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4533..4552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4786..4803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4818..4960
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4886..4905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5046..5068
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5062..5253)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(6062..7141,1..3))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7106..7125
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
       61 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
      121 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
      181 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
      241 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
      301 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
      361 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
      421 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg
      481 gatccatgcc ggagatccgc ctccgccatg tcgtgtcctg cagcagccag gactcgactc
      541 actgtgcaga aaatcttctc aaggcagaca cttaccgaaa atggcgggca gccaaggcag
      601 gcgagaagac catctctgtg gtcctacagt tggagaagga ggagcagata cacagtgtgg
      661 acattgggaa tgatggctca gctttcgtgg aggtgctggt gggcagttca gctggaggcg
      721 ctggggagca agactatgag gtccttctgg tcacctcatc tttcatgtcc ccttccgaga
      781 gccgcagtgg ctcaaacccc aaccgcgttc gcatgtttgg gcctgacaag ctggtccggg
      841 cagccgccga gaagcgctgg gaccgggtca aaattgtttg cagccagccc tacagcaagg
      901 actccccctt tggcttgagt tttgtacggt ttcatagccc cccagacaaa gatgaggcag
      961 aggccccgtc ccagaaggtg acagtgacca agcttggcca gttccgtgtg aaggaggagg
     1021 atgagagcgc caactctctg aggccggggg ctctcttctt cagccggatc aacaagacat
     1081 ccccagtcac agccagcgac ccggcaggac ctagctatgc agctgctacc ctccaggctt
     1141 ctagtgctgc ctcctcagcc tctccagtct ccagggccat aggcagcacc tccaagcccc
     1201 aggagtctcc caaagggaag aggaagttgg atttgaacca agaagaaaag aagaccccca
     1261 gcaaaccacc agcccagctg tcgccatctg ttcccaagag acctaaattg ccagctccaa
     1321 ctcgtacccc agccacagcc ccagtccctg cccgagcaca gggggcagtg acaggcaaac
     1381 cccgaggaga aggcaccgag cccagacgac cccgagctgg cccagaggag ctggggaaga
     1441 tccttcaggg tgtggtagtg gtgctgagtg gcttccagaa ccccttccgc tccgagctgc
     1501 gagataaggc cctagagctt ggggccaagt atcggccaga ctggacccgg gacagcacgc
     1561 acctcatctg tgcctttgcc aacaccccca agtacagcca ggtcctaggc ctgggaggcc
     1621 gcatcgtgcg taaggagtgg gtgctggact gtcaccgcat gcgtcggcgg ctgccctccc
     1681 ggaggtacct catggcaggg ccaggttcca gcagtgagga ggatgaggcc tctcacagcg
     1741 gtggcagcgg agatgaagcc cccaagcttc ctcagaagca accccagacc aaaaccaagc
     1801 ccactcaggc agctggaccc agctcacccc agaagccccc aacccctgaa gagaccaaag
     1861 cagcctcacc agtgctccag gaagatatag acattgaggg ggtacagtca gaaggacagg
     1921 acaatggggc ggaagattct ggggacacag aggatgagct gaggagggtg gcagagcaga
     1981 aggaacacag actgccccct ggccaggagg agaatgggga agacccgtat gcaggctcca
     2041 cggatgagaa cacggacagt gaggaacacc aggagcctcc tgatctgcca gtccctgagc
     2101 tcccagattt cttccagggc aagcacttct ttctttacgg ggagttccct ggggacgagc
     2161 ggcggaaact catccgatac gtcacagcct tcaatgggga gctcgaggac tatatgagtg
     2221 accgggttca gtttgtgatc acagcacagg aatgggatcc cagctttgag gaggccctga
     2281 tggacaaccc ctccctggca ttcgttcgtc cccgatggat ctacagttgc aatgagaagc
     2341 agaagttact tcctcaccag ctctatgggg tggtgccgca agccgtcgag caccaccacc
     2401 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     2461 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     2521 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
     2581 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2641 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2701 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2761 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2821 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2881 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2941 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3001 aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3061 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     3121 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     3181 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     3241 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     3301 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     3361 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     3421 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     3481 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     3541 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     3601 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     3661 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     3721 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     3781 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     3841 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     3901 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     3961 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4801 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4861 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4921 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4981 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5041 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     5101 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     5161 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     5221 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     5281 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     5341 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     5401 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     5461 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     5521 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     5581 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     5641 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     5701 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
     5761 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     5821 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     5881 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     5941 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     6001 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     6061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6121 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     6181 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     6241 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     6301 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     6361 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     6421 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     6481 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     6541 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     6601 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     6661 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     6721 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     6781 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     6841 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     6901 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     6961 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     7021 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     7081 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     7141 t
//
