LOCUS       sequence-126678-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for Hygromycin resistance gene plant expression
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..49)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    238..260
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
       61 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      181 aaccctgata aatgcttcaa taatgggacc gactcgcgct ctcctgagac ggatccgtta
      241 caccacaata tatcctgcca agatctaatt ccggggatcg gaaatccaga agcccgagag
      301 gttgccgcct ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc
      361 ggccggccgc cgggttgtgg gcaaaggcgc tcgacggtgg gcaaccgctt gcggttgtcc
      421 acgggcggag ccggtgcgcg tagcgcattg tccacaagcc aagggcgacc aataattgat
      481 atatatattc ataattgaaa agctaattga acatactact tgctgtaact acttgccgga
      541 gcgaggggtg tttgcaagct gttgatctga aagggctatt agcgttctca cgtgcctttt
      601 tgattagcga tttcacgtga ccttattagc gatttcacgt actccgatta gcgatttcac
      661 gtaccctgat tagcgatttc acgtggatag tttttggagc ggggccggaa agccccgtga
      721 atcaaggctt tgcggggcat tagcggtttc acgtggataa ctaccctcta tccacaggct
      781 tccggggata aaaa
//
