LOCUS       sequence-126697-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of murine Id2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-Id2
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(643..667)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(664..702)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    735..753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    777..795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agtcatcttt cacaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 atctgcagaa ttcggctttg ccatttattt agccacagag tactttgcta tcattcgaca
      121 taagctcaga agggaattca gatgcctgca aggacaggat gctgatgtcc gtgttcaggg
      181 tggtcagcgg cgtcctggac gcctggttct gtccaggtct ctggtgatgc aggctgacga
      241 tagtgggatg cgagtccagg gcgatctgca ggtccaagat gtaatcgatg acgtgctgca
      301 ggatttccat cttggtcacc ttcttgttct gggggatgct gggcaccagt tccttgagct
      361 tggagtagca gtcgttcatg ttgtagagca gactcatcgg gtcgtccacc ggggttttgc
      421 tccgggagat gcccaagctg tggtccgaca ggctgttttt cctaacggac ctcaccggac
      481 tgaaggcttt catgctgctc gtaggaaagc cgaattccag cacactggcg gccgttacta
      541 gtggatccga gctcggtacc aagctaattc ggggccgcgg aggctggatc ggtcccggtg
      601 tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa
      661 acgagctctg cttatatagg cctcccaccg tacacgccta cctcgacccg ggtaccgagc
      721 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      781 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      841 gtaaactcga ctttc
//
