LOCUS       sequence-126702-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA
  TITLE     The conserved npl4 protein complex mediates proteasome-dependent 
            membrane-bound transcription factor activation.
  JOURNAL   Mol Biol Cell 2001 Oct;12(10):3226-41.
  PUBMED    11598205
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2001-10"; volume: "12(10)"; pages: "3226-41"
COMMENT     Sequence Label: pPS2348
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..878
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 tggttgaaga acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaaccga
       61 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
      121 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
      181 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
      241 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
      301 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg
      361 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
      421 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
      481 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
      541 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
      601 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
      661 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
      721 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
      781 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
      841 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
      901 catgtatact aaactcacca aattagagct tcaatttaat tatatcaggt tattacccta
      961 tgcgggggtg aaataccgca cagatgcgtt aaggagaaaa ttaccgcatc agggcccttc
     1021 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg ccgggctcct cgcctattac
     1081 cgccagctgg cgaaa
//
