LOCUS       sequence-126709-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GST fusion vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Tanaka M, Gupta R, Mayer BJ
  TITLE     Differential inhibition of signaling pathways by dominant-negative 
            SH2/SH3 adapter proteins.
  JOURNAL   Mol Cell Biol. 1995 Dec . 15(12):6829-37.
  PUBMED    8524249
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1995 Dec . 15(12):6829-37."
COMMENT     Sequence Label: pEBG
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..702
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     660..682
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             709..726
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attctctaga gatccctcga cctcgagatc cattgtgctg gatctacaat ggcccctata
       61 ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt
      121 gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa
      181 aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa
      241 ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt
      301 tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac
      361 ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc
      421 aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat
      481 ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac
      541 atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa
      601 gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag
      661 ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct ggttccgcgt
      721 ggatccacta gtggtaccat cgatgcggcc gcgactctag agtgagggtc cccacctggg
      781 acccttgaga gtatcaggtc tcccacgtgg gagacaagaa atccctgttt aatatttaaa
      841 cagcagtgtt ccccatctgg gtccttgcac ccctcactct ggcctcagcc gactgcacag
      901 cgg
//
