LOCUS       sequence-12673-e       10116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by 
            human Synapsin I promoter for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester 
            I, Goshen I, Thompson KR, Deisseroth K
  TITLE     Molecular and cellular approaches for diversifying and extending 
            optogenetics.
  JOURNAL   Cell. 2010 Apr 2. 141(1):154-65.
  PUBMED    20303157
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 2. 141(1):154-65."
COMMENT     Sequence Label: pLenti-hSyn-eNpHR 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..10116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(4043..4045,4046..4756)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4085..4106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4346..4365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4693..4714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4808..5396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4861..4881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5279..5290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5399..5415)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5400..5416)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5921..6101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6127..6144)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6133..6357
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6403..6831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6490..6509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6700..6721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6840..6860)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6845..7174
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7025..7160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7087..7106
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7222..7269
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7288..7662
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7792..7913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7829..7848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7883..7902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7962..7978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7962..7978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7975..7997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7986..8002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8010..8016,8017..8034,8035..8040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8055..8076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8193..8210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8364..8952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8444..8463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9123..9914,9915..9983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9746..9765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9984..10088)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaaccg gtaggccacc atgacagaga ccctgcctcc
     3121 cgtgaccgag agtgccgtgg cccttcaagc cgaggttacc caaagggagt tgttcgagtt
     3181 cgtgctgaac gaccctttgc ttgcaagcag tctctatatc aacatcgcac ttgcaggact
     3241 gagtatactg ctgttcgttt ttatgacccg aggactcgat gatccacggg caaaacttat
     3301 tgctgtgtca accatccttg tgcctgtcgt cagcattgcc tcctacactg gattggcgag
     3361 cggcctgaca atttccgttc ttgaaatgcc agcgggccat tttgcagaag gcagctcagt
     3421 gatgctggga ggagaagagg tagatggtgt agtcaccatg tggggacggt atctcacctg
     3481 ggcactttcc acgcccatga ttctcctcgc tctgggtctc ctggccggaa gcaatgctac
     3541 aaagctcttc acagctatca ctttcgatat cgctatgtgc gtgactggcc ttgccgcggc
     3601 cctgactacc tcctcccacc tcatgagatg gttctggtac gctatcagtt gtgcatgctt
     3661 tctggtggtc ttgtatatcc tgctggtgga gtgggcacag gacgccaaag ccgcgggaac
     3721 cgctgacatg ttcaataccc tgaagctgtt gacagtagtg atgtggctgg ggtatccaat
     3781 tgtgtgggct cttggagtcg agggtatcgc ggtgttgccc gttggggtga cgagctgggg
     3841 atattctttc ctggatatcg tggcaaagta cattttcgca ttcttgctcc tgaactatct
     3901 gacgtcaaac gaatctgtcg tgtccggcag cattttggat gttccatctg cttctgggac
     3961 cccggctgat gatgcggccg ccaagagcag gatcaccagc gagggcgagt acatccccct
     4021 ggaccagatc gacatcaacg tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4081 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4141 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4201 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     4261 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4321 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4381 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4441 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4501 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4561 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4621 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4681 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4741 ggacgagctg tacaagttct gctacgagaa cgaggtgtaa tgagaattcg atatcaagct
     4801 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4861 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4921 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4981 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5041 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5101 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5161 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5221 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5281 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5341 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5401 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     5461 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     5521 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     5641 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     5701 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     5761 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     5821 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     5881 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     5941 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6001 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6061 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     6121 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6181 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6241 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6301 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6361 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     6421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     6841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7081 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7141 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7201 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7261 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     7321 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     7381 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     7441 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     7501 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     7561 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     7621 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     7681 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7741 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7921 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10081 gttccgcgca catttccccg aaaagtgcca cctgac
//
