LOCUS       sequence-12677-e        9979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: pPIGA-PHD
FEATURES             Location/Qualifiers
     source          1..9979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2215..2248)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2259..2758
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2294..2317)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2691..2710
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2776..3801
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2776..3801
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(4105..4122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4111..4335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4342..4375)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6208..6227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6209..6227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6234..6251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6234..6250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6243..6265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6391..6819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6478..6497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6688..6709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7163..7957
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7217..7236)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7827..7846
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7975..8043,8044..8835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8193..8212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9006..9594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9495..9514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9748..9765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9882..9903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9918..9923,9924..9941,9942..9948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9956..9972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9961..9979,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttggtac cgagctcgga tccactagta
       61 acggccgcca gtgtgctgga attcgccctt catggtacct tagcctagag tagatagcaa
      121 gttggagctg aggagaatag ctctttactg taaggctttc ttacttggtt tgaatgatga
      181 attccagtat ataagatgaa ctcatatgtg aatggaagtt ctaaaaactt atctctaaaa
      241 attgtcttat acattttcca ttgaaacatg agatgtttgc gacagtcaaa catttttgac
      301 tgaagcattt tgagacattc tgaccttcta caagtcttgt actatctctc ccatatgaaa
      361 catggaaagt tggagtctag tgcttacctg ttgggagtac atgtcagagt ccccgggcag
      421 ccttttcaga ctctctctac tccatcttga tgtccccctg cctccccaac cactccctag
      481 attccagtgt tatcctttag gagcagagat agaattgggg gtataattgg cagggatatt
      541 tggtcagaca ggttattttg atgataccat cctgatctcc actccctttg agaaccatgg
      601 cactgttaag ttgtgacttg accttcttca ttgacatcgt ataaacccca gaagcctgag
      661 ccactgtagg taataagtta acagggaaag aactgtggtg acggtttgtc agcatatagt
      721 aaaggggcta attgggattt ttttggtaac cacatttttc ttttggttaa agtaatagta
      781 cagcatctgt atttttttta attctatggt gatttataat acacttttag aaaaaagtaa
      841 atccttgcct ataatggatt gtcatcgcta acattctctt cataatagta aattgttatt
      901 ttatcactta aaactataag tagattaatt gacctgaaat aaagctggac aaaaaagtca
      961 gtttgtagtt actcttttaa ccctatgaca ttttaattca tttcaactcc tccttcttag
     1021 tttaactgtg ctatttagga aatcgatcat gaaattaatt tggagttcat gctgtaaaca
     1081 tacttcttca attgcaattc accagtctca gcataattct caacataatt ctgattaaaa
     1141 tgtttgtatt cagaatgtcc tcagggtaaa agagctcgat gaaaacatgc cgtacctttc
     1201 aagggctgtt gacctgtaca acatgcactg catgttataa cttccttgag ttcaaaagga
     1261 gacggtttaa aggtctaata tatgagacta agcacaagta atactcaaat tacaaaatat
     1321 gaatgttggc ccatttgatg tgttctgaac aagtatgttt aaagatcttc gttttttgat
     1381 attttagagc ttagtactta gcagtgtttt aaaaagcaag ccacctactt gctcagcaaa
     1441 aaacgttatt tcatttgcca gcatttgacc tatactatct actgaaattt cccccatctc
     1501 tttatcttct tttatttgaa atccatgaaa tgcttctgca tgtcctcacc atccatgctc
     1561 agcaacatgt acagcctcat gtggctgttg aaagaggagg ctctgtgttc ccagaagtat
     1621 gctagattgt agctctctgc ttgtggttga tgtctgccag atgccggtga gagcatctca
     1681 gcttagtcaa ggaatcttca aaggcaaatc ctgcttcagt actgctgaag tcactgctga
     1741 attaaatatt gaccctacag tggtgacccc aaaagctgtt cagactttgc gggactttgg
     1801 aattctagta gttggctaaa attgatggtg actccctagg ggagaggaac tgtggcttat
     1861 tctttgtatc acacagcctc tagcccggtg tcttgcccag aataagtacc caataagttt
     1921 tgaatcaaat ggcaataatt atcgtgatta agttcattaa ggatatcatt actatgacaa
     1981 caacaacaaa acacaagcaa cttttgtttg tatttgttta taaaatgttc ctgcaggatc
     2041 gggtcattgt ttatcatggg acaggtgatg gggtgatggg tgaaagtgct cacacttggc
     2101 ttctctccct ccaggtatat gaccgggtat cagtggaagc tgtgttgcca atggacaaac
     2161 gactggacag acttatttct cactgcggcc cagtaacagg ctacatcagc tagcataact
     2221 tcgtatagca tacattatac gaagttatga attctaccgg gtaggggagg cgcttttccc
     2281 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     2341 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     2401 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     2461 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     2521 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     2581 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     2641 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     2701 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     2761 ctgcatcccg ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2821 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     2881 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2941 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     3001 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     3061 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     3121 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     3181 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     3241 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     3301 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     3361 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     3421 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3481 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3541 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3601 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3661 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3721 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3781 cgtccgaggg caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc
     3841 ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg
     3901 gtggcacagt tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg
     3961 tcgcagtgat tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca
     4021 cgcactcggg tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt
     4081 gggtgcagat ccgtcgagtt aacgcctcga ctgtgccttc tagttgccag ccatctgttg
     4141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4321 cggtgggctc tatgggatat cataacttcg tatagcatac attatacgaa gttatgctag
     4381 ctttgttggc agttttcaac ttcctcttcc tcattttctt gagatggatg actccagatt
     4441 ctatcattga tgttgcaata gatgccactg ggccacgggg tgcctggact aataactatt
     4501 ctcacagtaa aagagggggt gagaataatg agatatctga aaccaggtag aaggaagcct
     4561 agattgtaag attttaaaca tttgtaatag ttctataaag actatggaaa ataaccttgc
     4621 ttttgggggg tttttgtttt tttagagtta atttagtaag ttatgctacc tctatatcat
     4681 tcaatatttt ctgttgagga aagataaaaa tgtatgcaat tcctgagtgt agaaacttct
     4741 tgcacttatt taaaatttag gagagaacat ttaagccact caggtatgca atttttcaga
     4801 ctactgaaat ccctgtagca gagatgtttt aacattatat tttgagagct ttgggtgctg
     4861 aagggccaaa cgttttctgg gcattttttg gccagttttt aatgtaacac cattagacac
     4921 tcaccagatg tttacaagtt ttctttaggg gaactacaac aattatatga actgttttat
     4981 atcatgttca tatacattta ttaggaatct aaatcatgtc tttgaacatt tattaggttc
     5041 actcagtagg tgttacatgt aattaacagg ttccttgagt aagatagtcc atcagttacc
     5101 agcacatttt gaacccctgc tctgtgtaga atgttgaact agatgcttcc cgccattaag
     5161 gaccaggggt gcattcactc tttgtttacc attcaaatgg cttacttcat cataattgtg
     5221 gttgatatga gatcaatatc caacatgcca aaaatgctca tgccagttaa tgccaggaaa
     5281 aaaatcaccg acacactact agtactttgt tcctgttgta tgcattctcc taggtagagc
     5341 ctccatcttc agttgtgttt gtgaaggtat tttttgcttt ttaaatactg gggaccgata
     5401 tcactgttga tagtgcagag aaaccctcca catttttcag tgcataattg agttttctat
     5461 aaatgccttc gtgttttctg agcagaatgt acgaggtgtg ccatcccaaa accagctgct
     5521 accctgtcct tttaatgtaa gtcactcccc ttcactgtgg cctcgctgat gtctgataag
     5581 tattgtcagt gtgcaaaagg ctttacttca gaatggttta tttatagcaa actaagttga
     5641 aaattttaga aacagtcttt gtgggtggat gttattaact gtcattgttg ttgcccagag
     5701 ccatgggttt tttaacccca aattatccac atggtgtgta ttatgaattc tttgaactct
     5761 taaggttttt gtgagaaaag gactgtgaat tcaaaacaat aaggcacttg tgggtgcact
     5821 acatagattc tgacagtgtt gtgattctgt ataggatttt taaaaatgac aacattcaca
     5881 aaatttatta ctttttaaaa aataacatgc ctattaactg gttgcactga tataaaagaa
     5941 atatatttgt gttttgtttg tactaaaatg caaaagcaag agtgcaattt ttaaaatcta
     6001 gaagttaggg gttttgttgg agaaaaatgg actgatcttt aaactattca gtcttactgg
     6061 gatttttatg catagaaact cacatataaa catgaaataa acagtgccag tattcatagg
     6121 aaagtgagaa actgtaatat ttggccatta ttctattcaa caggttttag aggcatgctc
     6181 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     6241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6361 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     6421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatt cagggcgcaa
     6841 gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg
     6901 gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca
     6961 ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag
     7021 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
     7081 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga
     7141 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     7201 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7261 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7321 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     7381 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7441 attgggcgaa gtgccggggc aggatctcct gtcatcccac cttgctcctg ccgagaaagt
     7501 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7561 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7621 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7681 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7741 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7801 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7861 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7921 catcgccttc tatcgccttc ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt
     7981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8881 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9781 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9841 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9901 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9961 agcggataac aatttcaca
//
