LOCUS       sequence-12689-e        6982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP112
FEATURES             Location/Qualifiers
     source          1..6982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(440..473)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3201..3234)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3764..3780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3764..3780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3777..3799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3788..3804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3812..3818,3819..3836,3837..3842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3857..3878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3995..4012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4166..4754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4246..4265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4925..5716,5717..5785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5548..5567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(5810..6057)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     6023..6041
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     6955..6973
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccggga taacttcgta tagcatacat tatacgaagt tatgggccca
      481 ttatgaatac gcacctctat gtatttccga gatacgatta ctccagttcc tcttacaaga
      541 aatgcataaa aatagttaca attaattaga acaagaactt atttagaaca cgttcacact
      601 gagtaagaac tcttgtccct tattagcctt gatagtgctg aaaaaaagaa aaaaaacaaa
      661 aaaaagaaat aaaataacgg caaacagcaa aggccacaga accgtattca tgttacttct
      721 gcaatatcaa tcacttactg gcaagtgcgt ataaattaaa cctatttctt tatcatcata
      781 tttacttata tctttaacag attccaaacc ctaaagtgtc cgaattttca atagggcgaa
      841 cttgaagaat aaccaaggtc aataatatat cttttagtat aaccctgaaa tttgccctat
      901 agaaatctag ggtttctgtg tggtttccgg gtgagtcata cggctttttt gaatttcttt
      961 ttttgcagtt gtctctatca atgaaaattt cgaggaagac gataaggtta agataagtag
     1021 ataagagaat gatacgagat aaagcacaaa ttagcagaaa gaagagtggt tgcgaacaga
     1081 gtaaaccgaa tcagggaatc cctttttgca aaaacatcaa ttatcctttt ctttttttta
     1141 cgtatatatc tggaacagaa atatataagt tactattata cttatagttg gatccagttt
     1201 ttaatctgtc gtcaatcgaa agtttatttc agagttcttc agacttctta actcctgtaa
     1261 aaacaaaaaa aaaaaaaggc atagcaatga caaattcaaa agaagacgcc gacatagagg
     1321 agaagcatat gtacaatgag ccggtcacaa ccctctttca cgacgttgaa gcttcacaaa
     1381 cacaccacag acgtgggtca ataccattga aagatgagaa aagtaaagaa ttgtatccat
     1441 tgcgctcttt cccgacgaga gtaaatggcg aggatacgtt ctctatggag gatggcatag
     1501 gtgatgaaga tgaaggagaa gtacagaacg ctgaagtgaa gagagagctt aagcaaagac
     1561 atattggtat gattgccctt ggtggtacta ttggtacagg tcttttcatt ggtttatcca
     1621 cacctctgac caacgccggc ccagtgggcg ctcttatatc atatttattt atgggttctt
     1681 tggcatattc tgtcacgcag tccttgggtg aaatggctac attcatccct gttacatcct
     1741 ctttcacagt tttctcacaa agattccttt ctccagcatt tggtgcggcc aatggttaca
     1801 tgtattggtt ttcttgggca atcacttttg ccctggaact tagtgtagtt ggccaagtca
     1861 ttcaattttg gacgtacaaa gttccactgg cggcatggat tagtattttt tgggtaatta
     1921 tcacaataat gaacttgttc cctgtcaaat attacggtga attcgagttc tgggtcgctt
     1981 ccatcaaagt tttagccatt atcgggtttc taatatactg tttttgtatg gtttgtggtg
     2041 ctggggttac cggcccagtt ggattccgtt attggagaaa cccaggtgcc tggggtccag
     2101 gtataatatc taaggataaa aacgaaggga ggttcttagg ttgggtttcc tctttgatta
     2161 acgctgcctt cacatttcaa ggtactgaac tcgttggtat cactgctggt gaagctgcaa
     2221 accccagaaa atccgttcca agagccatca aaaaagttgt tttccgtatc ttaaccttct
     2281 acattggctc tctattattc attggacttt tagttccata caatgaccct aaactaacac
     2341 aatctacttc ctacgtttct acttctccct ttattattgc tattgagaac tctggtacaa
     2401 aggttttgcc acatatcttc aacgctgtta tcttaacaac cattatttct gccgcaaatt
     2461 caaatattta cgttggttcc cgtattttat ttggtctatc aaagaacaag ttggctccta
     2521 aattcctgtc aaggaccacc aaaggtggtg ttccatacat tgcagttttc gttactgctg
     2581 catttggcgc tttggcttac atggagacat ctactggtgg tgacaaagtt ttcgaatggc
     2641 tattaaatat cactggtgtt gcaggctttt ttgcatggtt atttatctca atctcgcaca
     2701 tcagatttat gcaagctttg aaataccgtg gcatctctcg tgacgagtta ccatttaaag
     2761 ctaaattaat gcccggcttg gcttattatg cggccacatt tatgacgatc attatcatta
     2821 ttcaaggttt cacggctttt gcaccaaaat tcaatggtgt tagctttgct gccgcctata
     2881 tctctatttt cctgttctta gctgtttgga tcttatttca atgcatattc agatgcagat
     2941 ttatttggaa gattggagat gtcgacatcg attccgatag aagagacatt gaggcaattg
     3001 tatgggaaga tcatgaacca aagacttttt gggacaaatt ttggaatgtt gtagcataga
     3061 tatgacgttt tattaccttt gatcacattt ccacgccatt tcgcattctc accctcataa
     3121 gtcatacacc gaaaagaaag tttaagggat caatgagctt actataatct cagtatattt
     3181 atttttatcg atgattcacc ataacttcgt atagcataca ttatacgaag ttatcccggg
     3241 taccgagctc gaattcgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
     3301 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
     3361 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
     3421 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
     3481 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
     3541 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
     3601 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
     3661 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
     3721 aattataatt atttttatag cacgtgatga attcgtaatc atggtcatag ctgtttcctg
     3781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4921 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5761 cgacacggaa atgttgaata ctcatactct tcctttttca atattggccg caaattaaag
     5821 ccttcgagcg tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca
     5881 cgcgtctgta cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca
     5941 taactataaa aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg
     6001 ttagagcgga tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc
     6061 gagtgcgcag atatcactag tatatttgtt gtaaaaagta gataattact tccttgatga
     6121 tctgtaaaaa agagaaaaag aaagcatcta agaacttgaa aaactacgaa ttagaaaaga
     6181 ccaaatatgt atttcttgca ttgaccaatt tatgcaagtt tatatatatg taaatgtaag
     6241 tttcacgagg ttctactaaa ctaaaccacc cccttggtta gaagaaaaga gtgtgtgaga
     6301 acaggctgtt gttgtcacac gattcggaca attctgtttg aaagagagag agtaacagta
     6361 cgatcgaacg aactttgctc tggagatcac agtgggcatc atagcatgtg gtactaaacc
     6421 ctttcccgcc attccagaac cttcgattgc ttgttacaaa acctgtgagc cgtcgctagg
     6481 accttgttgt gtgacgaaat tggaagctgc aatcaatagg aagacaggaa gtcgagcgtg
     6541 tctgggtttt ttcagttttg ttctttttgc aaacaaatca cgagcgacgg taatttcttt
     6601 ctcgataaga ggccacgtgc tttatgaggg taacatcaat tcaagaagga gggaaacact
     6661 tcctttttct ggccctgata atagtatgag ggtgaagcca aaataaagga ttcgcgccca
     6721 aatcggcatc tttaaatgca ggtatgcgat agttcctcac tctttcctta ctcacgagta
     6781 attcttgcaa atgcctaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6901 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     6961 cgtatcacga ggccctttcg tc
//
