LOCUS       sequence-126951-        3744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF4C01
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2772..2780
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggcgtcac atcagtacca ccaaattgcc aatttgctgg aaaaaatgac gtcgacggac
       61 aaggacttcc gtttcatggc gacaaacgat ctgatgacgg agctacagaa ggatagcatc
      121 atcctggacg acgagtccga gaaaaaggtg gtgcgaatgg tgctcaagct gctggaagac
      181 aagaacggcg aagttcagaa cctggctgtc aagtgcctgg gtcccctggt caacaaggtg
      241 aaggagatcc aggtggagac catcgtggac tcgctgtgcg ccaacatgat gtccaacacg
      301 gagcagctgc gcgacatatc gagtattggc ctaaagacag tgattgccga gctgccgcag
      361 tcctcaaact ccctggcccc aaatgtctgt caaaggatca ccggcaaact gagcacagca
      421 attgagaagg aggacgtctc tgtgaaactg gagtcgctgg acattctggc cgatctgctc
      481 tctcgcttcg gggagttcct cgttcccttc cacagcacca ttctgaaagc tctgatgccc
      541 cagttggcct catcgcgcca ggctgttcgc aagcgtacca tcgtggccct gtcatttctg
      601 ctgatccagg ccaatagcaa cgcctacaac ggtgtcatcg atcatctgct cgatggccta
      661 gagaacccac caaatccagc tgccattcga acttacattc agtgcctggc ttcgatatgt
      721 cgacaggctg gccatcgact ttgcaatcac attgatcgct ccatgctcct gctgagccag
      781 tacagccagc gggatgacga tgagctgcgc gagttttgtc tgcaggcctg cgaagctttc
      841 gtcatgcgct gcccagatgc cataaacccg catattccca tgatcctgga gctgtgcctc
      901 aactatatca cctacgatcc aaactacaac tacgagacgg atgatggcga cactggcaat
      961 gccatggaca cggaggatga tgagtacgtc gatagtgagg agtacagtga cgacgacgac
     1021 atgagctgga aggttcgtcg cgctgcagcc aagtgtctgg aggtcttgat atccacacgg
     1081 caggagctcg tcgaggactt ctatcgcagc ctgagtccgg ctttgattgc gcgcttcaag
     1141 gagcgtgagg agaatgttaa gtccgacata tttcacgcct atgtggccct gctaaagaac
     1201 acacgcctca ccgacgacgt ggccaacgac cacgactcaa tggatcaggt atcgggtccg
     1261 acctcgctgt taattgagca gctgccactc attgtgaaag ccatacagcc gttaatgagg
     1321 gaaaaatcga tgaaaacgcg tcaagactgc ttcctcttgc tgcgcgagct gttgatttcg
     1381 ctaccaggtg ccctgggacc ctacttggac agcattgtgc caggcataag ctattcgctg
     1441 aatgacaaaa gctctaccag caacatgaag atcgaatctc tgggattcct ttactcgctg
     1501 ctccaaggac atccgccgca tgtctttcat ccacatattc ccctactggt gcccctcgta
     1561 gtgacttcgg tcttcgatcc tttctataag attgccaccg aggcgctttt ggtgctgcag
     1621 cagttggtaa aggtgattcg tccattagaa ccgaatgcgg ccaagtccga ctttgatgcc
     1681 ccatcgtttg tgggtcaggt ttactcgtgc acattgcaga aactgaaagt gacggatgtc
     1741 gaccaggagg taaaggagcg agccatagcc tgcatggggc agattatcgc caacatgggc
     1801 gacatgctgc agaatgagct ggcggtttgc ctgccaatct tcatggagcg cctaaagaac
     1861 gaagtaaccc gtttgagcag cgtgaaggcg ttgaccctga tcgcagcttc gtccctgcgc
     1921 atagatctga cacccatcct gcacgacgtg ttgcccgcat tgggcacatt cctgcgtaag
     1981 aaccaccgcg ccctcaagct gcactccctc gatctgatca acaagatcgt catcaactat
     2041 tcgagcaact tcgaggcgaa ccttctgcag acagccatag ttgagatccc accgcttata
     2101 tcggattcgg atttgcacgt agcccagtac tctctgacgc ttttgagcac cgtagctcgc
     2161 aggcagcccc aagctctggt cggcatacat gagcagttct tgcgctccgt gctcattttg
     2221 gttcgatctc ctttgctgca gggatccgca ttgaattgca cgttggagct gttccaggcc
     2281 ttggtgcaaa cacagctgtc tggcctggac tatcattcgc tcgtttcgaa gctaatggca
     2341 ccagttctgg gtggcaacgg tgacgtcaaa tccagagcca ctgccggcgc tcccagtgag
     2401 gtggtgcagc tgcacaagca ggcctatcac tcatcggcca agtgcattgc tgccttaaca
     2461 cagcagtgtc cccaggtggc gaccccattg gccacaaagc tgataacgga tctgcagaag
     2521 cgtaacgata cggaaatcat attctgtttg ctcaccatcg gtgagattgg caggcacttt
     2581 gatctgagct ccatacaggt gctgccgcag acaattattg aatgctttgg ggccacctcg
     2641 gaggatgtaa aggctgcagc ctcgcacgct ctcggcgctg tgtccgtggg cagcctgcag
     2701 acatacctgc ctctgattct gcacgagatt gaggtgcagc cgaagagaca gtatttgctg
     2761 ctacattcgc ttaaggaggt catttcatcg ctatcggtat cgcccagcgg actcgcccag
     2821 ctcctaccct cggtgccatc catttgggat cagctcttca agcattgcga gtgttcggag
     2881 gagggatcac gcaacgtggt ggccgagtgc ctgggcaaac tggtgctggt caatcccgac
     2941 gagctgctgc cgcaactgca gcaggcattg cgttccgaaa gcgccaccat gcgcaccgtt
     3001 gttgtctcct cagtaaagtt taccatctcc gatcagccgc agcccattga tgttctgctc
     3061 aagcagaaca ttggcgagtt tctatttgcc ctgcgagatc cggagcccca agtgagacgc
     3121 gtcgcactag tagccttcaa ttcggcggta cacaacaagc ccagtttggt gcgcgatttg
     3181 ctgcccaccc ttttgccttg gctctactcg gagacgaagg tgaagagcga actgattagg
     3241 gaggtggaga tgggtccctt caagcacacc gtcgacgacg gtctcgacat tcgcaaagcg
     3301 gcatttgaat gcatgtacac acttctcgag cagggcttgg atcgtgtgga tgtcatgcag
     3361 ttcctggacc atgtccaggc tggtctgtgc gaccactacg acatcaagat gctgacgtac
     3421 ctgatgacgg cacgtctggc catcttgtgc ccggataagg ttctgctaag actcgatcaa
     3481 tttatacagc aactacgtga tacgtgcacc cacaaggtga aggccaactc tgtgaaacag
     3541 gagtacgaaa agcaggacga actcaagcgt tctgcccttc gtgcagtctc ggccctttcg
     3601 caaataccca aagcaaacaa aaatcagcag ttagtggatt tcctcaagtc gatcaaagaa
     3661 acaccggaat taaacaagat cttcgagtac atacagaagg attccatcac cggtagctca
     3721 gatattatag taatggatca gagt
//
